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Sample GSM1302177 Query DataSets for GSM1302177
Status Public on Feb 11, 2016
Title 36 haim-rep2
Sample type RNA
 
Source name infecting hyphae of F.graminearum for maize stalk
Organism Fusarium graminearum
Characteristics time point (hours after inoculation): 36
Treatment protocol The maize tissue was fixed and paraffin embedded. Then Laser capture microdissection was used for material collection
Growth protocol Fusarium graminearum hyphae infecting maize stalk
Extracted molecule total RNA
Extraction protocol Picopure RNA isolation kit (Acturus) was used for trace RNA extraction
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from infecting hyphae for maize stalk
Data processing The data were analyzed with software R, the RMA package
 
Submission date Jan 07, 2014
Last update date Feb 11, 2016
Contact name Weihua Tang
Organization name Shanghai Inst. Plant Phys. & Eco.
Lab Plant-Fungal Interaction & Plant Reproductive Biology
Street address 300 Fenglin Rd
City Shanghai
ZIP/Postal code 200032
Country China
 
Platform ID GPL18136
Series (1)
GSE53854 Gene expression of F.graminearum during maize stalk infection

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
1415670_at 2.93731284
1415671_at 2.335016141
1415672_at 2.014939449
1415673_at 2.040418684
1415674_a_at 3.446493103
1415675_at 3.526965751
1415676_a_at 3.164065849
1415677_at 2.677346504
1415678_at 3.225102679
1415679_at 3.033630911
1415680_at 2.901039204
1415681_at 2.824602384
1415682_at 2.508396171
1415683_at 2.651045906
1415684_at 2.073832416
1415685_at 2.07858522
1415686_at 2.467701759
1415687_a_at 2.694828143
1415688_at 3.054615727
1415689_s_at 2.299275303

Total number of rows: 18069

Table truncated, full table size 447 Kbytes.




Supplementary file Size Download File type/resource
GSM1302177_CB2007190-E2.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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