|
| Status |
Public on Feb 11, 2016 |
| Title |
144 haim-rep2 |
| Sample type |
RNA |
| |
|
| Source name |
infecting hyphae of F.graminearum for maize stalk
|
| Organism |
Fusarium graminearum |
| Characteristics |
time point (hours after inoculation): 144
|
| Treatment protocol |
The maize tissue was fixed and paraffin embedded. Then Laser capture microdissection was used for material collection
|
| Growth protocol |
Fusarium graminearum hyphae infecting maize stalk
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Picopure RNA isolation kit (Acturus) was used for trace RNA extraction
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from infecting hyphae for maize stalk
|
| Data processing |
The data were analyzed with software R, the RMA package
|
| |
|
| Submission date |
Jan 07, 2014 |
| Last update date |
Feb 11, 2016 |
| Contact name |
Weihua Tang |
| Organization name |
Shanghai Inst. Plant Phys. & Eco.
|
| Lab |
Plant-Fungal Interaction & Plant Reproductive Biology
|
| Street address |
300 Fenglin Rd
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platform ID |
GPL18136 |
| Series (1) |
| GSE53854 |
Gene expression of F.graminearum during maize stalk infection |
|
