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Sample GSM130280 Query DataSets for GSM130280
Status Public on Aug 23, 2007
Title Lymphatic endothelial cells upon conditioned medium from NSCLC 24 h
Sample type RNA
 
Source name Dermal microvascular cells, commercially available
Organism Homo sapiens
Characteristics RNA Pooled from 6 different individuals
Biomaterial provider CAMBREX
Treatment protocol Conditioned medium from lung carcinoma cells growth under 20% oxigen, 24 h
Growth protocol BEM w/o bFGF and VEGF supplemented with concentrated supernatants from growth media derived from lung adenocarcinoma cells grown under normoxia
Extracted molecule total RNA
Extraction protocol Trizol and mini RNeasy kit (Qiagen)
Label Biotin
Label protocol IVT labeling kit from affymetrix
 
Hybridization protocol Hybridization: According to the manufacturer's protocol (Affymetrix).
Scan protocol Scanner: GeneChip Scanner 3000.
Description - Normalization: Employing GCOS software, chips were normalized using all probe sets scaling option and target signal at 500.
Data processing Microarray suite 5.0, Tiling Array
 
Submission date Aug 23, 2006
Last update date Aug 28, 2018
Contact name Ana Rouzaut
E-mail(s) arouzaut@unav.es
Phone 34 948194700
Fax 34 948194714
Organization name University of Navarra/CIMA
Department Oncology
Lab 1.03
Street address Pio XII 55
City Pamplona
State/province Navarra
ZIP/Postal code 31008
Country Spain
 
Platform ID GPL570
Series (1)
GSE5579 Hypoxia and lymphatic endothelial cells
Relations
Reanalyzed by GSE64985
Reanalyzed by GSE86362
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE mean signal intensity
ABS_CALL P present, M marginal, A absent
DETECTION P-VALUE p value for call; present: less than 0.05, marginal: 0.05-0.065, absent: more than 0.065

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 101.1 P 0.000754
AFFX-BioB-M_at 165.6 P 0.000044
AFFX-BioB-3_at 87.9 P 0.000110
AFFX-BioC-5_at 279.3 P 0.000044
AFFX-BioC-3_at 334.2 P 0.000044
AFFX-BioDn-5_at 647.1 P 0.000044
AFFX-BioDn-3_at 1459.5 P 0.000070
AFFX-CreX-5_at 3168.5 P 0.000044
AFFX-CreX-3_at 4002.2 P 0.000044
AFFX-DapX-5_at 81.6 P 0.000060
AFFX-DapX-M_at 157.6 P 0.000662
AFFX-DapX-3_at 246.5 P 0.000081
AFFX-LysX-5_at 10.1 P 0.016655
AFFX-LysX-M_at 19.8 A 0.131361
AFFX-LysX-3_at 27.0 P 0.000081
AFFX-PheX-5_at 26.5 P 0.000095
AFFX-PheX-M_at 28.8 P 0.003212
AFFX-PheX-3_at 25.4 P 0.007616
AFFX-ThrX-5_at 16.9 P 0.000857
AFFX-ThrX-M_at 31.3 P 0.000110

Total number of rows: 54675

Table truncated, full table size 1417 Kbytes.




Supplementary file Size Download File type/resource
GSM130280.CEL.gz 7.9 Mb (ftp)(http) CEL
GSM130280.EXP.gz 497 b (ftp)(http) EXP

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