|
Status |
Public on Aug 23, 2007 |
Title |
Lymphatic endothelial cells upon conditioned medium from NSCLC 24 h |
Sample type |
RNA |
|
|
Source name |
Dermal microvascular cells, commercially available
|
Organism |
Homo sapiens |
Characteristics |
RNA Pooled from 6 different individuals
|
Biomaterial provider |
CAMBREX
|
Treatment protocol |
Conditioned medium from lung carcinoma cells growth under 20% oxigen, 24 h
|
Growth protocol |
BEM w/o bFGF and VEGF supplemented with concentrated supernatants from growth media derived from lung adenocarcinoma cells grown under normoxia
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol and mini RNeasy kit (Qiagen)
|
Label |
Biotin
|
Label protocol |
IVT labeling kit from affymetrix
|
|
|
Hybridization protocol |
Hybridization: According to the manufacturer's protocol (Affymetrix).
|
Scan protocol |
Scanner: GeneChip Scanner 3000.
|
Description |
- Normalization: Employing GCOS software, chips were normalized using all probe sets scaling option and target signal at 500.
|
Data processing |
Microarray suite 5.0, Tiling Array
|
|
|
Submission date |
Aug 23, 2006 |
Last update date |
Aug 28, 2018 |
Contact name |
Ana Rouzaut |
E-mail(s) |
arouzaut@unav.es
|
Phone |
34 948194700
|
Fax |
34 948194714
|
Organization name |
University of Navarra/CIMA
|
Department |
Oncology
|
Lab |
1.03
|
Street address |
Pio XII 55
|
City |
Pamplona |
State/province |
Navarra |
ZIP/Postal code |
31008 |
Country |
Spain |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE5579 |
Hypoxia and lymphatic endothelial cells |
|
Relations |
Reanalyzed by |
GSE64985 |
Reanalyzed by |
GSE86362 |
Reanalyzed by |
GSE119087 |