|
| Status |
Public on Jan 09, 2014 |
| Title |
DRG RVS5 |
| Sample type |
RNA |
| |
|
| Source name |
vincristine saline
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: sprague-dawley
age: 3 months
tissue: dorsal root ganglion
|
| Treatment protocol |
10 days of vincristine/saline treatment to induce neuropathic pain followed by 5 days of morphine/oxycodone or saline treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Standard Illumina label protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
All manipulations and statistical analyses were performed with the R freeware. For Illumina beadchip data, all spots with a detection p-value greater than 0.05% were excluded. A two-way ANOVA was performed, using vincristine treatment as the first factor and analgesic treatment as the second factor.
|
| |
|
| Submission date |
Jan 08, 2014 |
| Last update date |
Jan 09, 2014 |
| Contact name |
Karine Thibault |
| Organization name |
ESPCI
|
| Lab |
Laboratoire de Neurobiologie
|
| Street address |
10 rue Vauquelin
|
| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platform ID |
GPL6101 |
| Series (1) |
| GSE53897 |
Genome-wide analysis of dorsal root ganglion (DRG) gene expression after morphine or oxycodone treatment |
|
