|
| Status |
Public on Dec 31, 2014 |
| Title |
TAX, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
tumor treated by TAX
|
| Organism |
Homo sapiens |
| Characteristics |
treatment (of tumor-bearing female balb/c nude mice): Taxotere
tissue: tumor
|
| Treatment protocol |
Mice bearing similar volume of tumors were chosen and divided into four groups randomly with 6 mice in each group. Mice from different groups were treated with 10 mg/kg of TAX, DSNs, equivalent BSNs or glucose (GLU) separately by tail vein injection with the frequency of every 4 days. Body weight and tumor size were measured every 3 days. Mice were sacrificed on day 12 (D12) and tumor tissues were isolated and frozen in liquid nitrogen immediately for RNA isolation
|
| Growth protocol |
Tumor suppression effects of DSNs and TAX were investigated with tumor-bearing female BALB/c nude mice in JOINN Laboratories (Beijing, China). BALB/c nude mice were purchased from Vital River Laboratories (Beijing, China). Mice were subcutaneous injected with MCF-7 cells. After three weeks, the tumor bearing mice were sacrificed for further experiments. The tumor tissues were isolated and cut into pieces at about 2 mm3 under aseptic conditions. Around 4-5 weeks old mice were subcutaneously inoculated with tumor tissues on the right flank. Tumors were allowed to grow to about 50-100 mm3.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized with Genechip Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned with Genechip Scanner 3000
|
| Description |
TAX_r2
tumor bearing mice were treated with 10 mg/kg of Taxotere (TAX) by tail vein injection with the frequency of every 4 days. Mice were sacrificed on day 12 (D12) and tumor tissues were isolated.
|
| Data processing |
The expression raw data was preprocessed using Robust Multichip Analysis (RMA) with quantile normalization and custom CDF file hgu133plus2hsentrezgcdf, v17.1.0
|
| |
|
| Submission date |
Jan 15, 2014 |
| Last update date |
Dec 31, 2014 |
| Contact name |
Ying Ge |
| E-mail(s) |
geying.tju@gmail.com
|
| Organization name |
Dalian Medical University
|
| Street address |
9 Western Section, Lvshun South Street
|
| City |
Dalian |
| ZIP/Postal code |
116044 |
| Country |
China |
| |
|
| Platform ID |
GPL17811 |
| Series (1) |
| GSE54091 |
Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity |
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