|
| Status |
Public on May 14, 2015 |
| Title |
Rhode Island Red (RIR) |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Blood
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: blood
breed: Rhode Island Red (RIR)
gender: Female
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated using standard phenol/chloroform extraction methods.
|
| Label |
Cy5
|
| Label protocol |
1.5 ug of test DNA were labeled with Cy5 and 1.5 ug of reference DNA were labeled with Cy3 using Agilent Genomic DNA Labeling Kit PLUS.
|
| |
|
| Channel 2 |
| Source name |
Blood
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: blood
breed: Red Jungle Fowl
gender: Female
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated using standard phenol/chloroform extraction methods.
|
| Label |
Cy3
|
| Label protocol |
1.5 ug of test DNA were labeled with Cy5 and 1.5 ug of reference DNA were labeled with Cy3 using Agilent Genomic DNA Labeling Kit PLUS.
|
| |
|
| |
| Hybridization protocol |
Array hybridization was performed using the Agilent Oligo aCGH Hybridization Kit for 40 h at 20 rpm in a 65℃ Agilent hybridization oven.
|
| Scan protocol |
Agilent arrays were scanned at 2 μm resolution using an Agilent scanner with 90% laser power value and PMT 100%.
|
| Data processing |
Agilent Feature Extraction 9.5 Software was used for spot quality filtering and normalization. Log2 ratio values were calculated from default log10 ratio values between tests and reference.
|
| |
|
| Submission date |
Jan 15, 2014 |
| Last update date |
May 14, 2015 |
| Contact name |
Guoqiang Yi |
| E-mail(s) |
yiguoqiang20@hotmail.com
|
| Phone |
+8662731351
|
| Organization name |
Agricultural Genome Institute at Shenzhen
|
| Street address |
No 7,Pengfei Road,Dapeng District,Shenzhen
|
| City |
Shenzhen |
| State/province |
Guangdong |
| ZIP/Postal code |
518120 |
| Country |
China |
| |
|
| Platform ID |
GPL18175 |
| Series (2) |
| GSE54118 |
Validation experiment of copy number variations identified by next-generation sequencing in chickens (part 2) |
| GSE54119 |
Validation experiment of copy number variations identified by next-generation sequencing in chickens |
|
