|
Status |
Public on May 14, 2015 |
Title |
White Leghorn (WL) |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
Blood
|
Organism |
Gallus gallus |
Characteristics |
tissue: blood breed: White Leghorn (WL) gender: Female
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was isolated using standard phenol/chloroform extraction methods.
|
Label |
Cy5
|
Label protocol |
1.5 ug of test DNA were labeled with Cy5 and 1.5 ug of reference DNA were labeled with Cy3 using Agilent Genomic DNA Labeling Kit PLUS.
|
|
|
Channel 2 |
Source name |
Blood
|
Organism |
Gallus gallus |
Characteristics |
tissue: blood breed: Red Jungle Fowl gender: Female
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was isolated using standard phenol/chloroform extraction methods.
|
Label |
Cy3
|
Label protocol |
1.5 ug of test DNA were labeled with Cy5 and 1.5 ug of reference DNA were labeled with Cy3 using Agilent Genomic DNA Labeling Kit PLUS.
|
|
|
|
Hybridization protocol |
Array hybridization was performed using the Agilent Oligo aCGH Hybridization Kit for 40 h at 20 rpm in a 65℃ Agilent hybridization oven.
|
Scan protocol |
Agilent arrays were scanned at 2 μm resolution using an Agilent scanner with 90% laser power value and PMT 100%.
|
Data processing |
Agilent Feature Extraction 9.5 Software was used for spot quality filtering and normalization. Log2 ratio values were calculated from default log10 ratio values between tests and reference.
|
|
|
Submission date |
Jan 15, 2014 |
Last update date |
May 14, 2015 |
Contact name |
Guoqiang Yi |
E-mail(s) |
yiguoqiang20@hotmail.com
|
Phone |
+8662731351
|
Organization name |
Agricultural Genome Institute at Shenzhen
|
Street address |
No 7,Pengfei Road,Dapeng District,Shenzhen
|
City |
Shenzhen |
State/province |
Guangdong |
ZIP/Postal code |
518120 |
Country |
China |
|
|
Platform ID |
GPL18175 |
Series (2) |
GSE54118 |
Validation experiment of copy number variations identified by next-generation sequencing in chickens (part 2) |
GSE54119 |
Validation experiment of copy number variations identified by next-generation sequencing in chickens |
|