|
| Status |
Public on Jul 26, 2014 |
| Title |
fer-1_96h S. enterica SL1344_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
fer-1, infected, 96h
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: HH142
allele: fer-1(b232ts)
hours: 96h
diet: S. enterica SL1344
condition: infected
|
| Treatment protocol |
For recovery, tetracycline was included in the plate at 50ug/ml.
|
| Growth protocol |
Synchronized L1 fer-1(b232ts) C. elegans animals grown on E. coli OP50 for 36 hours or S. enterica SL1344 for 36, 72, 96, and 120 hours. Synchronized L1 fer-1(b232ts) C. elegans animals grown on S. enterica SL1344 for 72 hours and then shifted to E. coli HT115 plus Tetracycline plates. Synchronized L1 fer-1(b232ts) C. elegans animals grown on S. enterica SL1344 for 96 hours and then shifted to E. coli HT115 plus Tetracycline plates. All animals grown at 25dC.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol (Life Technologies) and the RNeasy Plus Universal Kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
100ng of total RNA was converted to 1.65ug Cy-3-labeled, linearly amplified cRNA using the Low Input Quick Amp (LIQA) One-Color Microarray-Based Gene Expression Analysis Kit (Agilent)
|
| |
|
| Hybridization protocol |
Hybridization was performed in the Agilent Rotisserie Hybridization Oven (Agilent) for 17 hours.
|
| Scan protocol |
Agilent B scanner according to standard Agilent protocols (Agilent).
|
| Data processing |
Log2 transformation and quantile normalization using Partek Genomics Suite (Partek).
|
| |
|
| Submission date |
Jan 17, 2014 |
| Last update date |
Jul 26, 2014 |
| Contact name |
Brian P Head |
| E-mail(s) |
brian.head@dm.duke.edu
|
| Phone |
919-668-2449
|
| Organization name |
Duke University Medical Center
|
| Department |
Molecular Genetics and Microbiology
|
| Lab |
Alejandro Aballay
|
| Street address |
Box 3580 DUMC
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL11346 |
| Series (1) |
| GSE54212 |
C. elegans gene expression in response to Salmonella enterica infection and recovery by Tetracycline treatment |
|
