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Sample GSM1311626

Query DataSets for GSM1311626

Status

Public on Jun 01, 2015

Title

x203

Sample type

RNA

Channel 1

Source name

Breast tumor tissue

Organism

Characteristics

group: Sporadic
pam50agilent: LumA

Extracted molecule

total RNA

Extraction protocol

Total RNA was extracted from freshly frozen tumor tissue using Trizol Reagent (Invitrogen) and RNeasy Micro Kit (Qiagen).

Label

Cy5

Label protocol

RNA was amplified and labeled using the Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion) according to the manufacturer’s protocol.

Channel 2

Source name

Universal Human Reference RNA (Stratagene)

Organism

Characteristics

reference: Universal Human Reference RNA (Stratagene)

Extracted molecule

total RNA

Extraction protocol

Total RNA was extracted from freshly frozen tumor tissue using Trizol Reagent (Invitrogen) and RNeasy Micro Kit (Qiagen).

Label

Cy3

Label protocol

RNA was amplified and labeled using the Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion) according to the manufacturer’s protocol.

Hybridization protocol

Fragmentation, hybridization and washing for the spotted arrays were carried out using Agilent Gene Expression Hybridization Kit (Agilent Technologies ) and Gene Expression Wash Buffer Kit (Agilent Technologies) according to the manufacturer’s protocol in a low ozone environment.

Scan protocol

Scanned using a Agilent G2565CA Microarray scanner

Description

K176

Data processing

Scanned images were quantified by Gene Pix Pro 6.0 (Molecular Devices). Bad quality features flagged during feature extraction were removed and the remaining data were pre-processed. Data were background corrected (normexp, offset=50), then within-array normalized by loess normalization method and between-array normalizatized by the quantile method. The normalized values were used to calculate log2 transformed Cy5/Cy3 ratios. Replicate probes were collapsed by calculating the median. The 92 samples were processed together with 154 other breast tumor samples. ComBat method was used for adjustment of the batch effects across the different spotting batches [PMID: 16632515]. In cases of multiple probes per gene symbol only the probe with the maximum mean (Cy5) intensity was kept. Missing expression values were imputed by k-nearest neighbors averaging (k = 10).

Submission date

Jan 22, 2014

Last update date

Jun 01, 2015

Contact name

Martin Jakob Larsen

E-mail(s)

martin.larsen@rsyd.dk

Organization name

Odense University Hospital

Department

Dept. of Clinical Genetics

Street address

Sdr. Boulevard 29

City

Odense C

ZIP/Postal code

5000

Country

Denmark

Platform ID

Series (1)

Microarray gene expression analysis: Batch effect removal improves the cross-platform consistency

Data table header descriptions
ID_REF
VALUE	Log2 transformed ratios(Cy5/Cy3) representing test/reference

Data table
ID_REF	VALUE
CGEN_HUMAN_LIB2_6010321789_0	1.4867558823126
CGEN_HUMAN_LIB2_6010305554_0	-0.989393800114107
CGEN_HUMAN_LIB2_6010316348_0	-0.0486786913923928
CGEN_HUMAN_LIB2_6010318548_0	0.154217429849304
CGEN_HUMAN_LIB2_6010322653_0	0.491736052810246
CGEN_HUMAN_LIB2_6010306515_0	0.227170772100963
CGEN_HUMAN_LIB2_6010327270_0	-1.17220405761151
CGEN_HUMAN_LIB2_6010305889_0	0.718526890763954
CGEN_HUMAN_LIB2_6010322217_0	-0.306215707808027
CGEN_HUMAN_LIB2_6010300416_0	-1.23010189128614
CGEN_HUMAN_LIB2_6010328195_0	4.65743375663834
CGEN_HUMAN_LIB2_6010311510_0	-0.0902766433912302
CGEN_HUMAN_LIB2_6010306064_0	-0.789875684886785
CGEN_HUMAN_LIB2_6010325142_0	-0.0836840412920184
CGEN_HUMAN_LIB2_6010326970_0	1.48340334729546
CGEN_HUMAN_LIB2_6010302501_0	-0.935038890289584
CGEN_HUMAN_LIB2_6010308530_0	-0.927807413826187
CGEN_HUMAN_LIB2_6010328062_0	0.292108606208302
CGEN_HUMAN_LIB2_6010328856_0	0.109560533896716
CGEN_HUMAN_LIB2_6010313980_0	-0.289071311774281

Total number of rows: 28919

Table truncated, full table size 1332 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM1311626_x203.gpr.gz	2.7 Mb	(ftp)(http)	GPR
Processed data included within Sample table

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