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Sample GSM1314256 Query DataSets for GSM1314256
Status Public on Apr 07, 2015
Title TRACK 43-49 kidney 1
Sample type SRA
 
Source name kidney cortex
Organism Mus musculus
Characteristics tissue: kidney cortex
strain: C57BL/6
gender: male
genotype: TRACK/gamma-HIF1alphaM3 TG+
Treatment protocol No treatment
Growth protocol Animals were kept at standard conditions in Research Animal Resource Center of WCMC
Extracted molecule total RNA
Extraction protocol Thin outer slice of kidney cortex was immediately removed from the animals. mRNA was extracted from the kidney cortex slice using mini-RNAeasy columns (Qiagen, Valencia, CA).
illumina TruSeq RNA Sample Preparation kit
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing Reads were aligned to the genome using the Burrows-Wheeler Aligner in Goby web software (goby_1.9.8.3 (20120216154520)), using default parameters.
Differential Expression Analysis were done with Differential Expression Analysis with Goby in Goby web software (goby_1.9.8.3 (20120216154520)), using default parameters.
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using Goby Web software.
Genome_build: NCBI37.55/MM9
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample
 
Submission date Jan 24, 2014
Last update date May 15, 2019
Contact name Leiping Fu
E-mail(s) lef2010@med.cornell.edu
Phone 212-746-6262
Organization name Weill Cornell Medical College
Department Pharmacology
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL13112
Series (1)
GSE54390 mRNA profiling of GGT-HIF1aM3 (γ-HIF1αM3, TRACK) kidney cortex
Relations
BioSample SAMN02596008
SRA SRX449478

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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