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Status |
Public on Aug 08, 2018 |
Title |
1-B6-TGFbeta |
Sample type |
RNA |
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|
Source name |
B6-TGFbeta
|
Organism |
Mus musculus |
Characteristics |
genotype/variation: B6-TGFß (WT) disease state: induced renal fibrosis
|
Treatment protocol |
Mice were maintained in a pathogen-free facility, with 4–5 animals per cage in a temperature-controlled room with a 12-h light/dark cycle and access to food and water ad libitum. Age and sex matched littermate cohorts were used for all the experiments to account for potential strain differences.
|
Growth protocol |
All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
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Data processing |
RMA by software Partek
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|
Submission date |
Jan 27, 2014 |
Last update date |
Aug 08, 2018 |
Contact name |
WeiPing Chen |
E-mail(s) |
weipingChen@niddk.nih.gov
|
Phone |
301-496-0175
|
Organization name |
NIDDK/NIH
|
Department |
GCL
|
Lab |
Genomics Core Lab
|
Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL10740 |
Series (1) |
GSE54441 |
Differences among inbred mouse strains in sensitivity to TGF-ß1 induced renal fibrosis: association with TIMP-1 and EGR2 expression |
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