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Sample GSM1315403 Query DataSets for GSM1315403
Status Public on Aug 08, 2018
Title 1-B6-TGFbeta
Sample type RNA
 
Source name B6-TGFbeta
Organism Mus musculus
Characteristics genotype/variation: B6-TGFß (WT)
disease state: induced renal fibrosis
Treatment protocol Mice were maintained in a pathogen-free facility, with 4–5 animals per cage in a temperature-controlled room with a 12-h light/dark cycle and access to food and water ad libitum. Age and sex matched littermate cohorts were used for all the experiments to account for potential strain differences.
Growth protocol All animal studies were approved by the NIDDK/NIH Animal Care and Use Committee.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Data processing RMA by software Partek
 
Submission date Jan 27, 2014
Last update date Aug 08, 2018
Contact name WeiPing Chen
E-mail(s) weipingChen@niddk.nih.gov
Phone 301-496-0175
Organization name NIDDK/NIH
Department GCL
Lab Genomics Core Lab
Street address Bldg 8, Room 1A11, NIDDK/NIH
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL10740
Series (1)
GSE54441 Differences among inbred mouse strains in sensitivity to TGF-ß1 induced renal fibrosis: association with TIMP-1 and EGR2 expression

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10338001 12.4974
10338002 5.73532
10338003 11.1293
10338004 10.5531
10338005 2.86873
10338006 3.10564
10338007 3.46216
10338008 3.96046
10338009 6.68734
10338010 2.89946
10338011 5.43462
10338012 2.9891
10338013 2.68176
10338014 2.79162
10338015 2.74281
10338016 6.48595
10338017 13.7401
10338018 6.08651
10338019 5.01728
10338020 6.98673

Total number of rows: 241576

Table truncated, full table size 3984 Kbytes.




Supplementary file Size Download File type/resource
GSM1315403_02-14-13_J_Kopp_SS_1_Mo_Gene1_0_ST_1_P1_B6_TGFbeta.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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