|
| Status |
Public on Jan 31, 2014 |
| Title |
Macrophages_P1heart_3daysMI_1 |
| Sample type |
RNA |
| |
|
| Source name |
cardiac macrophages, P1, 3 days post-MI
|
| Organism |
Mus musculus |
| Characteristics |
age at mi: P1
strain: ICR
cell type: CD11b+Ly6G- cells sorted from heart
gender: mixed
|
| Treatment protocol |
no treatments applied
|
| Growth protocol |
All animals were maintained in compliance with the Institutional Animal Care and Use Committee at University of Texas Southwestern Medical Center
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from mouse tissues and cell lines using Promega ReliaPrep RNA cell system according to the manufacturer’s protocol
|
| Label |
Cy3
|
| Label protocol |
cRNA was generated and labeled according to the protocols outlined at: http://microarray.swmed.edu/protocols/protocol/illumina-gene-expression-probe-labeling/
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
Illumina bead studio software was used to analyze with quantile normalization
|
| |
|
| Submission date |
Jan 30, 2014 |
| Last update date |
Jan 31, 2014 |
| Contact name |
Arin B Aurora |
| E-mail(s) |
arinaurora@gmail.com
|
| Organization name |
UT Southwestern Medical Center
|
| Department |
Molecular Biology
|
| Lab |
Eric N. Olson
|
| Street address |
6000 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75235 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE54530 |
Comparison of gene expression in cardiac macrophages from P1 or P14 mice 3 days following MI |
|
