|
| Status |
Public on Sep 07, 2014 |
| Title |
Dog-MC-401188 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
DNA from mammary tumor tissue of dog 401188
|
| Organism |
Canis lupus familiaris |
| Characteristics |
strain/breed: unknown
sample id: dog 401188
tissue: canine mammary cancer (MC)
tissue subtype: Simple carcinoma
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from the dissected tissues using a Qiagen Tissue DNeasy purification kit (exactly following the kit manual)
|
| Label |
Cy5
|
| Label protocol |
Labeling test samples with Cy3 following the NimbleGen official Manual, see more at NimbleGen website, and labeling reference samples with Cy5 following the NimbleGen official Manual, see more at NimbleGen website.
|
| |
|
| Channel 2 |
| Source name |
DNA from mammary normal tissue of dog 401188
|
| Organism |
Canis lupus familiaris |
| Characteristics |
strain/breed: unknown
dog id: dog 401188
tissue: mammary normal tissue
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from the dissected tissues using a Qiagen Tissue DNeasy purification kit (exactly following the kit manual)
|
| Label |
Cy3
|
| Label protocol |
Labeling test samples with Cy3 following the NimbleGen official Manual, see more at NimbleGen website, and labeling reference samples with Cy5 following the NimbleGen official Manual, see more at NimbleGen website.
|
| |
|
| |
| Hybridization protocol |
Using NimbleGen Hybridization System (following the NimbleGen official Manual)
|
| Scan protocol |
Scanning two-color NimbleGen arrays with GenePix 4000B Scanner and associated software(following the NimbleGen official Manual)
|
| Description |
MC-401188
|
| Data processing |
Analyzing the data with the segMNT algorithm (following the NimbleGen official Manual), and background correction as implemented in the NimbleScan software package, version 2.6.0.0 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Jan 30, 2014 |
| Last update date |
Sep 08, 2014 |
| Contact name |
Deli Liu |
| E-mail(s) |
del2017@med.cornell.edu
|
| Organization name |
Weill Cornell Medicine
|
| Street address |
1300 York Ave
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL9314 |
| Series (1) |
| GSE54535 |
Molecular homology and difference between spontaneous canine mammary cancer and human breast cancer. |
|
