|
Status |
Public on Jun 03, 2014 |
Title |
MPN patient sample (MPD_3) |
Sample type |
RNA |
|
|
Source name |
MPN patient sample, Peripheral blood neutrophils
|
Organism |
Homo sapiens |
Characteristics |
cell type: Peripheral blood neutrophils diagnosis: PV jak2 v617f quantitative genotyping: WT_inferred calr status: WT tet2 status: WT mpl w515k/l status: na jak2 amp(9p24.3): na del(20q13.11): na
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was purified from granulocytes using Trizol (Invitrogen).
|
Label |
biotin
|
Label protocol |
Linear amplification of 20 ng of total RNA was performed using the Ovation Biotin RNA Amplification and Labeling System (Nugen)
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|
|
Hybridization protocol |
The cDNA was fragmented, labeled and hybridized to Affymetrix HG_U133AAofAv2 microarrays (Affymetrix, Santa Clara, CA)
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Scan protocol |
Standard Affymetrix protocol
|
Description |
Gene expression data from peripheral blood granulocytes from a patient with chronic MPN.
|
Data processing |
Raw expression values were normalized using Robust Multiarray Averaging (RMA) using quantile normalization
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|
|
Submission date |
Feb 04, 2014 |
Last update date |
Jun 03, 2014 |
Contact name |
Fatima Al-Shahrour |
E-mail(s) |
shahrour@broadinstitute.org
|
Organization name |
Broad Institute of MIT and Harvard
|
Department |
Cancer program
|
Street address |
7 Cambridge Center
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02142 |
Country |
USA |
|
|
Platform ID |
GPL4685 |
Series (2) |
GSE54644 |
Integrated genomic analysis illustrates the central role of JAK-STAT pathway activation in myeloproliferative neoplasm pathogenesis [MPN patients] |
GSE54646 |
Integrated genomic analysis illustrates the central role of JAK-STAT pathway activation in myeloproliferative neoplasm pathogenesis |
|