|
| Status |
Public on Jun 03, 2014 |
| Title |
HEL_JAK2 knockdown (Jak2-10 shRNA)_3 |
| Sample type |
RNA |
| |
|
| Source name |
HEL cell line_JAK2 knockdown (Jak2-10 shRNA)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: JAK2V617F-mutant AML cell line HEL
treatment: JAK2 knockdown (Jak2-10 shRNA)
|
| Treatment protocol |
The JAK2V617F-mutant AML cell line HEL was treated with 2 independent shRNA lentiviruses for JAK2 in a pLKO-puromycin selectable vector as well as 2 non-targeting shRNA controls (sh-Luciferase and GFP vector control).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified from HEL cells using Trizol (Invitrogen)
|
| Label |
biotin
|
| Label protocol |
Linear amplification of 20 ng of total RNA was performed using the Ovation Biotin RNA Amplification and Labeling System (Nugen)
|
| |
|
| Hybridization protocol |
The cDNA was fragmented, labeled and hybridized to Affymetrix HG_U133AAofAv2 microarrays (Affymetrix, Santa Clara, CA)
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
Replicate 3 Jak2-10 shRNA
|
| Data processing |
Raw expression values were normalized using Robust Multiarray Averaging (RMA) using quantile normalization
|
| |
|
| Submission date |
Feb 04, 2014 |
| Last update date |
Jun 03, 2014 |
| Contact name |
Fatima Al-Shahrour |
| E-mail(s) |
shahrour@broadinstitute.org
|
| Organization name |
Broad Institute of MIT and Harvard
|
| Department |
Cancer program
|
| Street address |
7 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL4685 |
| Series (2) |
| GSE54645 |
Integrated genomic analysis illustrates the central role of JAK-STAT pathway activation in myeloproliferative neoplasm pathogenesis [HEL cell lines] |
| GSE54646 |
Integrated genomic analysis illustrates the central role of JAK-STAT pathway activation in myeloproliferative neoplasm pathogenesis |
|
