 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 01, 2015 |
| Title |
MS240_1 with array type Medgene -1.0stv1 from Affymetrix |
| Sample type |
RNA |
| |
|
| Source name |
MS240_1
|
| Organism |
Medicago truncatula |
| Characteristics |
genotype/variation: mutant (ms240)
age: 14 days
dev. stage (boyes et al. plant cell 2001): nodule
|
| Treatment protocol |
no treatment
|
| Growth protocol |
roots - In vitro in 12cm square plates using the BN medium. 16h day light, 24 °C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
MS240_1:6ug.
|
| Label |
Biotin
|
| Label protocol |
labelling Biotin direct, amplification=yes, total RNA 50 ng.
|
| |
|
| Hybridization protocol |
labelled extract quantity: 5ug
|
| Scan protocol |
GCOS, Biotin:pmt voltage 570nm,650V,laser power 1
|
| Description |
The experiment is designed to detect transcriptome differences between wild type and two fix minus (NF737 and MS240) mutant lines.
|
| Data processing |
The data were normalized with the gcrma algorithm (Irizarry et al., 2003), available in the Bioconductor package (Gentleman and Carey, 2002).
|
| |
|
| Submission date |
Feb 11, 2014 |
| Last update date |
Dec 01, 2015 |
| Contact name |
labrune cecile |
| E-mail(s) |
labrune@evry.inra.fr
|
| Organization name |
INRA
|
| Street address |
2 rue gaston cremieux
|
| City |
evry |
| ZIP/Postal code |
91000 |
| Country |
France |
| |
|
| Platform ID |
GPL18240 |
| Series (1) |
| GSE54862 |
affy_med_2013_05-Comparison between nodules of WT, NF737 and MS240 lines |
|
| Data table header descriptions |
| ID_REF |
ID number |
| VALUE |
Normalized data by GCRMA |
| Data table |
| ID_REF |
VALUE |
| 12300795 |
0.389475 |
| 12300796 |
0.672663 |
| 12300798 |
0.831760 |
| 12300799 |
1.311286 |
| 12300802 |
0.599377 |
| 12300807 |
1.114670 |
| 12300809 |
0.722099 |
| 12300811 |
1.006828 |
| 12300812 |
0.829180 |
| 12300814 |
2.021802 |
| 12300815 |
0.537360 |
| 12300816 |
1.104918 |
| 12300817 |
0.765239 |
| 12300818 |
0.877039 |
| 12300820 |
0.761691 |
| 12300822 |
1.076866 |
| 12300823 |
0.930933 |
| 12300825 |
0.934510 |
| 12300826 |
1.378865 |
| 12300827 |
0.821139 |
Total number of rows: 108723
Table truncated, full table size 1911 Kbytes.
| Supplementary file |
Size |
Download |
File type/resource |
| GSM1325310_MS240_1.CEL.gz |
3.7 Mb |
(ftp)(http) |
CEL |
| Processed data included within Sample table |
|
|
|
|
 |
