|
| Status |
Public on Apr 05, 2014 |
| Title |
KO LSK, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse bone marrow LSK
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Vav1-iCre x Cbfb(F/F)
cell type: LSK
mouse genetic background: C57BL6 N12 backcrossed
|
| Treatment protocol |
n/a
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
NucleoSpin RNA XS kit (Macherey-Nagel)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard NuGen WT-Ovation Pico RNA Amplification system
|
| |
|
| Hybridization protocol |
Following fragmentation with NuGen Encore Biotin Module,2.5 ug of cDNA were hybridized into the GeneChip Hybridization Oven 640 for 18 hr at 45C on Gene 1.0 ST Array. GeneChip were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip 7G 3000 Scanner
|
| Description |
Gene expression data from adult BM progenitors
|
| Data processing |
The data was analyzed with the Affymetrix GeneChip Command Console. Microarray expression data was processed using Command Console (Affymetrix, Inc) and the raw (.CEL) files generated were analyzed using Arraystar version 4 (DNAStar) software with Robust Multichip Analysis ( RMA).
|
| |
|
| Submission date |
Feb 20, 2014 |
| Last update date |
Apr 06, 2014 |
| Contact name |
Takeshi Egawa |
| E-mail(s) |
tegawa@wustl.edu
|
| Organization name |
Washington University School of Medicine
|
| Department |
Pathology and Immunology
|
| Street address |
660 South Euclid
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE55227 |
Gene expression analysis of Cbfb-deficient LSK and GMP |
|
