|
Status |
Public on Feb 21, 2014 |
Title |
Synovial tissue from osteoarthritic joint 5 (OA_5) |
Sample type |
RNA |
|
|
Source name |
synovial tissue from osteoarthritic joint
|
Organism |
Homo sapiens |
Characteristics |
disease state: synovial tissue isolated from osteoarthritic joint tissue: synovial tissue isolated from joint
|
Treatment protocol |
tissues were collected from joint replacement surgery in OA patients and synovecomy surgery in RA patients, normal synovium was collected early post mortem from macroscopically normal knee joints
|
Growth protocol |
tissues were immediately frozen in liquid nitrogen for subsequent RNA-extraction
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was collected by using the RNeasy mini kit (Qiagen)
|
Label |
biotin
|
Label protocol |
3-5 µg of total RNA was amplified and labelled using the GeneChip® one-cycle target labelling and control reagents (Affymetrix)
|
|
|
Hybridization protocol |
50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
Description |
Gene expression data of synovial tissue isolated from osteoarthritic joint number 5, labelled as OA_5
|
Data processing |
GCOS
|
|
|
Submission date |
Feb 21, 2014 |
Last update date |
Feb 21, 2014 |
Contact name |
Thomas Häupl |
E-mail(s) |
thomas.haeupl@charite.de
|
Phone |
+49 30 450513293
|
Organization name |
Charité
|
Department |
Rheumatology
|
Street address |
Charitéplatz 1
|
City |
Berlin |
ZIP/Postal code |
10117 |
Country |
Germany |
|
|
Platform ID |
GPL96 |
Series (1) |
GSE55235 |
Identification of rheumatoid arthritis and osteoarthritis patients by transcriptome-based rule set generation |
|