|
| Status |
Public on Feb 21, 2014 |
| Title |
Synovial tissue from osteoarthritic joint 5 (OA_5) |
| Sample type |
RNA |
| |
|
| Source name |
synovial tissue from osteoarthritic joint
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: synovial tissue isolated from osteoarthritic joint
tissue: synovial tissue isolated from joint
|
| Treatment protocol |
tissues were collected from joint replacement surgery in OA patients and synovecomy surgery in RA patients, normal synovium was collected early post mortem from macroscopically normal knee joints
|
| Growth protocol |
tissues were immediately frozen in liquid nitrogen for subsequent RNA-extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was collected by using the RNeasy mini kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
3-5 µg of total RNA was amplified and labelled using the GeneChip® one-cycle target labelling and control reagents (Affymetrix)
|
| |
|
| Hybridization protocol |
50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data of synovial tissue isolated from osteoarthritic joint number 5, labelled as OA_5
|
| Data processing |
GCOS
|
| |
|
| Submission date |
Feb 21, 2014 |
| Last update date |
Feb 21, 2014 |
| Contact name |
Thomas Häupl |
| E-mail(s) |
thomas.haeupl@charite.de
|
| Phone |
+49 30 450513293
|
| Organization name |
Charité
|
| Department |
Rheumatology
|
| Street address |
Charitéplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE55235 |
Identification of rheumatoid arthritis and osteoarthritis patients by transcriptome-based rule set generation |
|
