 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Feb 28, 2014 |
| Title |
89_PIT_pre-puberty |
| Sample type |
SRA |
| |
|
| Source name |
Pituitary gland
|
| Organism |
Bos taurus |
| Characteristics |
developmental stage: PRE-puberty
tissue: Pituitary gland
breed: Brangus (3/8 Brahman x 5/8 Angus)
|
| Treatment protocol |
Duplicate 10 g samples of each tissue were ground in liquid nitrogen with mortar and pestle.
|
| Growth protocol |
All tissues were collected within 15 min postmortem, snap-frozen in liquid nitrogen, and then stored at -80° C until processing.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using a Trizol protocol (Invitrogen, Carlsbad, CA).
mRNA was purified, fragmented, and converted to cDNA. Adapters were ligated to the ends of double-stranded cDNA and PCR amplified to create libraries. These procedures were executed with the TruSeq RNA Sample Preparation kit of Illumina (San Diego, CA).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Filtering for quality by NGS Quality Control tool using CLC Genomics Workbench (CLC Bio, Aarhus, Denmark).
RNA-Seq analysis using CLC Genomics Workbench Ver. 6.0
Genome_build: Annotated UMD3.1 bovine reference genome (http://www.ncbi.nlm.nih.gov/genome/?term=bos+taurus)
Supplementary_files_format_and_content: .csv containing Ensembl ID, Gene name, chromosome, gene start (bp), gene end (bp), average unique reads, RPKM means.
|
| |
|
| Submission date |
Feb 27, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Angela Cánovas |
| E-mail(s) |
acanovas@uoguelph.ca
|
| Organization name |
University of Guelph
|
| Department |
Animal Biosciences
|
| Lab |
Cánovas Lab
|
| Street address |
50 Stone Road East
|
| City |
Guelph |
| State/province |
ON |
| ZIP/Postal code |
N1G 2W1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL15749 |
| Series (1) |
| GSE55435 |
Multi-Tissue Omics Analyses Reveals Molecular Regulatory Networks for Puberty in Composite Beef Cattle |
|
| Relations |
| BioSample |
SAMN02666482 |
| SRA |
SRX477487 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
