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Sample GSM1338641 Query DataSets for GSM1338641
Status Public on Mar 04, 2014
Title 545060_A09_I317_circadien_B73_CRS_B73_0_cc24h
Sample type RNA
 
Source name maize_B73_circadian cycle_24h_light
Organism Zea mays subsp. mays
Characteristics genotype: standard maize line B73
phenotype: sampled after 24h in continuous light
tissue: leaf elongation zone
Growth protocol Well watered plants of the maize line B73 were previously grown (entrainement period) with 12h of photoperiod and with high day/night variations of evaporative demand in controlled conditions (day: 28°C, vapour pressure deficit of 3kPa and light 600µmol m-2 s-1; night: 20°C, vapour pressure deficit of 0.8kPa and darkness).
Leaf samples were collected at predawn (1h before lights on) and 12 and 24 hours in continuous light after light switched on.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the RNeasy Mini kit (Qiagen Inc., Valencia, CA, USA) and the RNAprotect reagent (Qiagen) and DNA was removed by on-column DNase digestion with the RNase-Free DNase set (Qiagen). RNA quality and concentration was determined by analysis with NanoDrop.
Label Cy3
Label protocol Labeling was performed using One-Color DNA Labeling 5223555001 kit, Roche NimbleGen Inc. following the standard operating protocol. See www.nimblegen.com.
 
Hybridization protocol Hybridization was performed using Nimblegen Hybridization system following the standard operating protocol. See www.nimblegen.com.
Scan protocol Scanning was performed with scanner MS200 Roche-NimbleGen with autogain mode, following the standard operating protocol. See www.nimblegen.com.
Description This sample of leaf elongation zone was collected from maize plants 24h after lights on. It is the second of three biological replicates used in this experiment, each from separate plants.
Data processing The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249)and quantile normalization (Bolstad et al. Bioinformatics 19(2):185) as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
 
Submission date Mar 03, 2014
Last update date Mar 04, 2014
Contact name Cecilio Frois Caldeira
E-mail(s) cecilio.frois@gmail.com
Organization name INRA - Institut National de la Recherche Agronomique
Department Herault
Lab LEPSE
Street address 2 place Viala
City Montpellier
ZIP/Postal code 34060
Country France
 
Platform ID GPL18328
Series (1)
GSE55524 Expression analysis of maize (Zea mays L.) genes in continuous light

Data table header descriptions
ID_REF
VALUE RMA-normalized, gene-level summarized signal intensity

Data table
ID_REF VALUE
AC147602_5_FGT002 1492.792901
AC147602_5_FGT003 1492.262701
AC147602_5_FGT004 425.0206
AC148152_3_FGT001 116.8691001
AC148152_3_FGT006 133.4751999
AC149475_2_FGT002 13493.65789
AC149633_4_FGT002 3203.614598
AC149633_4_FGT003 1188.411999
AC149633_4_FGT005 9328.136595
AC149810_2_FGT003 6515.221003
AC149818_2_FGT005 929.2044992
AC149818_2_FGT008 1610.327502
AC149818_2_FGT009 131.6288
AC149828_2_FGT003 330.3764004
AC149828_2_FGT004 2997.440403
AC149829_2_FGT002 5353.134594
AC149829_2_FGT004 561.5724995
AC152495_1_FGT002 518.0828999
AC152495_1_FGT003 2095.180199
AC152495_1_FGT010 2308.188601

Total number of rows: 44829

Table truncated, full table size 1078 Kbytes.




Supplementary file Size Download File type/resource
GSM1338641_545060_A09_DR01_2012-10-09_09-01_532_grid.pair.gz 2.8 Mb (ftp)(http) PAIR
GSM1338641_GEO_sample_24h_2.txt.gz 449.9 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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