|
| Status |
Public on Sep 01, 2006 |
| Title |
LDL-treatment, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
APRE-19 cells with LDL treatment
|
| Organism |
Homo sapiens |
| Characteristics |
nonimmortalized human RPE cell line ARPE-19
|
| Treatment protocol |
ARPE-19 cells were seeded at 100,000/cm2 in T-75 cm2 flasks containing Dulbecco’s Modified Eagle medium with 15 mM Hepes buffer (DMEM; Invitrogen-Gibco BRL, Gaithersberg, MD) and 10% fetal bovine serum (FBS; Invitrogen-Gibco BRL) and 2 mM L-glutamine solution, (Invitrogen-Gibco BRL) at 37oC for 1 week, and serum withdrawn in DMEM+1% bovine serum albumin (BSA) for 3 days to make ARPE-19 cells quiescent11.LDL (Intracel, Frederick, MD was added to the medium for 24 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Affymetrix G3000 GeneArray Scanner
|
| Description |
human APRE cell line APRE-19 cells with LDL treatment.
|
| Data processing |
Fluorescence was detected using the Affymetrix G3000 GeneArray Scanner and image analysis of each GeneChip was done through the GeneChip Operating System 1.1.1 (GCOS) software from Affymetrix, using the standard default settings.The details of data nalysis are described in paper.
|
| |
|
| Submission date |
Sep 01, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Yanqin Yang |
| Organization name |
The Johns Hopkins University
|
| Street address |
600 N. Wolfe Street
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21287 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE5741 |
Expression data from ARPE-19 cells treated with LDL or ox-LDL |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
