|
| Status |
Public on Jul 17, 2014 |
| Title |
C57BL/6J H3K4me1 HF Replicate 2 |
| Sample type |
SRA |
| |
|
| Source name |
Liver
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
chip antibody: Abcam, ab8895
tissue: liver
diet: High Fat
|
| Treatment protocol |
Mice were fed control or high fat diet
|
| Growth protocol |
Livers were harvested from humanely euthanized mice
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Libraries were prepared according to Illumina instructions for RNA-seq or DNA-seq
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
ChIP-seq
|
| Data processing |
ChIP-seq: ChIPs were performed with an anti-H3K4me1 antibody (Abcam, ab8895) using standard ChIP and our published protocols (Leung et al., 2013). Sequencing libraries compatible with Illumina HiSeq 2500 technology was generated using Illumina protocols. We obtained 55M million tags for livers of C57BL/6J control and 71M million reads for tags of C57BL/6J HF. Sequences were aligned to the mm9 reference genome as with FAIRE-seq tags.
ChIP-seq: BEDPE file format
Genome_build: mm9
|
| |
|
| Submission date |
Mar 04, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Amy Leung |
| Organization name |
Beckman Research Institute, City of Hope
|
| Department |
Diabetes/Cancer
|
| Lab |
Schones
|
| Street address |
1500 E. Duarte Rd
|
| City |
Duarte |
| ZIP/Postal code |
91010 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE55581 |
Diet-induced chromatin remodeling in mice livers |
|
| Relations |
| BioSample |
SAMN02673260 |
| SRA |
SRX481156 |
