|
| Status |
Public on May 29, 2015 |
| Title |
siTLX_IFNg_1 |
| Sample type |
RNA |
| |
|
| Source name |
U251 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
sirna transfection: siRNA targeting TLX
stimulation: IFN-gamma
cell type: astroglioma
cell line: U251
|
| Treatment protocol |
After cells had settled and adhered in 6-well plates, they were transfected with 10 nM siRNA targeting human TLX (or negative control siRNA targeting luciferase) using HiPerfect transfection reagent. 72 hours after transfection, cells were stimulated with 10ng/ml of recombinant IFN-gamma for 8 hours
|
| Growth protocol |
U251 cells were grown to subconfluency in 6-well plates with DMEM media supplemented with 10% serum and antibiotics.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from individual wells of 6-well plate using the Qiagen Rneasy Plus kit.
|
| Label |
biotin
|
| Label protocol |
Biotin labeled complementary RNA (cRNA) was made using the EpiCentre Nano Total-Amp kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were quantile normalized without background subtraction using Illumina GenomeStudio V1.8
|
| |
|
| Submission date |
Mar 10, 2014 |
| Last update date |
May 29, 2015 |
| Contact name |
daniel beiting |
| E-mail(s) |
beiting@vet.upenn.edu
|
| Organization name |
University of Pennsylvania
|
| Department |
Pathobiology
|
| Lab |
Beiting
|
| Street address |
380 S. University Ave
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE55750 |
A Gain-of-Function Genomic Screen Identifies the Orphan Nuclear Receptor TLX as an Enhancer of STAT1-mediated Transcription and Immunity to Toxoplasma gondii: TLX siRNA |
| GSE55751 |
A Gain-of-Function Genomic Screen Identifies the Orphan Nuclear Receptor TLX as an Enhancer of STAT1-mediated Transcription and Immunity to Toxoplasma gondii |
|
