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Status
Public on Sep 07, 2006
Title
Hela, mock, 24 hr vs. HeLa, 193-MAPK14, 24 hr, unmodified
Sample type
RNA
Channel 1
Source name
HeLa
Organism
Homo sapiens
Characteristics
cell line: HeLa conditions: mock, 24 hr
Extracted molecule
total RNA
Label
Cy3
Channel 2
Source name
HeLa
Organism
Homo sapiens
Characteristics
cell line: HeLa conditions: 193-MAPK14, 24 hr, unmodified
Extracted molecule
total RNA
Label
Cy5
Description
For details please see: Jackson AL, Burchard J, Leake D, Reynolds A, Schelter J, Guo J, Johnson JM, Lim L, Karpilow J, Nichols K, Marshall W, Kvorova A, and Linsley PS. RNA (2006) 12(7):1197-1205
Data processing
Local background correction
Submission date
Sep 01, 2006
Last update date
Jul 22, 2009
Contact name
Aimee Jackson
Organization name
Regulus Therapeutics
Street address
1896 Rutherford Rd
City
Carlsbad
State/province
CA
ZIP/Postal code
92008
Country
USA
Platform ID
GPL3992
Series (1)
GSE5769
Position-specific chemical modification of siRNAs reduces "off-target" transcript silencing
Data table header descriptions
ID_REF
Rosetta generated unique probe identifier
VALUE
same as UNF_VALUE but with flagged values removed: Corrected (normalized) Log10 Ratio of channels (CH2/CH1)
LOGINTENSITY
Corrected average log intensity of channels
INTENSITY1
Raw Cy3 intensity (CH1)
INTENSITY2
Raw Cy5 intensity (CH2)
PVALUE
P-value of LogRatio
QUALITY
1 - if good and non control, 0 - otherwise
UNF_VALUE
Corrected (normalized) Log10 Ratio of channels (CH2/CH1)
Data table
ID_REF
VALUE
LOGINTENSITY
INTENSITY1
INTENSITY2
PVALUE
QUALITY
UNF_VALUE
10023804644
0.0067
-2.2763
0.0053
0.0054
9.9166e-001
1
0.0067
10023804645
0.0400
-0.1426
0.6936
0.7709
5.1472e-001
1
0.0400
10023804646
-0.0251
-0.5164
0.3154
0.2973
6.1863e-001
1
-0.0251
10023804647
-0.0394
-1.5696
0.0282
0.0258
7.8569e-001
1
-0.0394
10023804648
0.0110
-0.4306
0.3678
0.3780
8.2974e-001
1
0.0110
10023804649
-0.0064
-1.5076
0.0313
0.0309
9.6243e-001
1
-0.0064
10023804650
0.0381
-1.8980
0.0120
0.0135
9.1300e-001
1
0.0381
10023804651
-0.0543
-1.9169
0.0132
0.0114
8.8391e-001
1
-0.0543
10023804652
-0.0994
-0.6138
0.2767
0.2210
7.8506e-002
1
-0.0994
10023804653
0.0040
-1.6130
0.0245
0.0245
9.8327e-001
1
0.0040
10023804654
0.0942
-2.1195
0.0068
0.0085
8.4326e-001
1
0.0942
10023804655
0.0465
0.2010
1.5114
1.6773
3.9316e-001
1
0.0465
10023804656
-0.0144
0.3252
2.1684
2.0920
7.6769e-001
1
-0.0144
10023804657
-0.0008
-0.0977
0.8133
0.8050
9.8794e-001
1
-0.0008
10023804658
0.0030
-1.6242
0.0237
0.0238
9.8471e-001
1
0.0030
10023804659
0.0174
0.3347
2.1202
2.2107
7.2938e-001
1
0.0174
10023804660
-0.0113
-1.1943
0.0654
0.0636
9.0737e-001
1
-0.0113
10023804661
0.0220
-1.2640
0.0544
0.0571
8.3982e-001
1
0.0220
10023804662
0.0606
1.1409
12.9067
14.9496
3.2240e-001
1
0.0606
10023804663
-0.0181
-1.1336
0.0758
0.0727
8.4617e-001
1
-0.0181
Total number of rows: 23570 Table truncated, full table size 1445 Kbytes .Supplementary data files not provided
Processed data included within Sample table