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Status
Public on Sep 07, 2006
Title
HeLa, mock, 24hr vs. HeLa, MAPK14-193, unmodified, 10nM, 24 hr
Sample type
RNA
Channel 1
Source name
HeLa
Organism
Homo sapiens
Characteristics
cell line: HeLa conditions: mock, 24hr
Extracted molecule
total RNA
Label
Cy3
Channel 2
Source name
HeLa
Organism
Homo sapiens
Characteristics
cell line: HeLa conditions: MAPK14-193, unmodified, 10nM, 24 hr
Extracted molecule
total RNA
Label
Cy5
Description
For details please see: Jackson AL, Burchard J, Leake D, Reynolds A, Schelter J, Guo J, Johnson JM, Lim L, Karpilow J, Nichols K, Marshall W, Kvorova A, and Linsley PS. RNA (2006) 12(7):1197-1205
Data processing
Local background correction
Submission date
Sep 01, 2006
Last update date
Jul 22, 2009
Contact name
Aimee Jackson
Organization name
Regulus Therapeutics
Street address
1896 Rutherford Rd
City
Carlsbad
State/province
CA
ZIP/Postal code
92008
Country
USA
Platform ID
GPL3992
Series (1)
GSE5769
Position-specific chemical modification of siRNAs reduces "off-target" transcript silencing
Data table header descriptions
ID_REF
Rosetta generated unique probe identifier
VALUE
same as UNF_VALUE but with flagged values removed: Corrected (normalized) Log10 Ratio of channels (CH2/CH1)
LOGINTENSITY
Corrected average log intensity of channels
INTENSITY1
Raw Cy3 intensity (CH1)
INTENSITY2
Raw Cy5 intensity (CH2)
PVALUE
P-value of LogRatio
QUALITY
1 - if good and non control, 0 - otherwise
UNF_VALUE
Corrected (normalized) Log10 Ratio of channels (CH2/CH1)
Data table
ID_REF
VALUE
LOGINTENSITY
INTENSITY1
INTENSITY2
PVALUE
QUALITY
UNF_VALUE
10023804644
-0.0639
-1.9203
0.0131
0.0114
6.8257e-001
1
-0.0639
10023804645
-0.0514
0.0630
1.2270
1.0925
3.4647e-001
1
-0.0514
10023804646
0.0546
-0.3562
0.4139
0.4700
2.6183e-001
1
0.0546
10023804647
0.0516
-1.4617
0.0326
0.0370
5.8544e-001
1
0.0516
10023804648
0.0538
-0.5312
0.2771
0.3141
2.6230e-001
1
0.0538
10023804649
0.0209
-1.4810
0.0324
0.0340
8.1104e-001
1
0.0209
10023804650
0.0379
-1.6458
0.0217
0.0237
5.9157e-001
1
0.0379
10023804651
-0.0182
-0.0459
0.9214
0.8837
6.7947e-001
1
-0.0182
10023804652
-0.0339
-0.9037
0.1304
0.1202
5.3737e-001
1
-0.0339
10023804653
0.0251
-1.4373
0.0362
0.0382
7.3253e-001
1
0.0251
10023804654
-0.0030
-1.9510
0.0116
0.0116
9.8561e-001
1
-0.0030
10023804655
0.0408
-0.0197
0.9122
1.0042
4.3037e-001
1
0.0408
10023804656
-0.0594
0.6759
5.0837
4.4441
2.5069e-001
1
-0.0594
10023804657
0.0157
-0.0870
0.8053
0.8339
7.5313e-001
1
0.0157
10023804658
0.0591
-1.9531
0.0107
0.0120
7.1738e-001
1
0.0591
10023804659
0.0491
0.5897
3.6826
4.1172
3.3051e-001
1
0.0491
10023804660
-0.0109
-1.1322
0.0767
0.0749
8.2684e-001
1
-0.0109
10023804661
0.0675
-0.8086
0.1454
0.1699
1.3759e-001
1
0.0675
10023804662
0.0018
0.9992
9.9600
10.0547
9.7800e-001
1
0.0018
10023804663
0.0183
-1.0143
0.0980
0.1001
8.0586e-001
1
0.0183
Total number of rows: 23570 Table truncated, full table size 1444 Kbytes .Supplementary data files not provided
Processed data included within Sample table