|
| Status |
Public on Sep 08, 2007 |
| Title |
ovarian gene expression innthe absence of FIGLA, an oocyte specific transcription factor |
| Sample type |
SAGE |
| Anchor |
Sau3A
|
| Tag Count |
79095 |
| Tag Length |
10 |
| |
|
| Source name |
normal Versus FIGLA null ovary at new born developmental stage
|
| Organism |
Mus musculus |
| Characteristics |
CF1 normal mice and mixed background Figla null mouse
Gender: female
age: newborn
homozygous Null mouse for Figla
Normal CF1
tissue: ovary
|
| Biomaterial provider |
CRL for normal mice
|
| Treatment protocol |
Figla null mouse line was made by targetted disruption of Figla gene in the mouse chromosome by homologous recombination in mouse 129Sv ES cells. The correctly targetted ES cells were injected in the blastocyst of C57BL/6 mice
|
| Growth protocol |
not applicable
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
total RNA was extracted from the normal and homozygous null mice by using RNeasy mini kit (Qiagen, Valencia, CA). homozygous null mouse RNA was confired by one step RT PCR using primers for ZP2 and MSY gene and absence of ZP2 and presence of MSY gene was considered as homozygous null ovary RNA. Poly (A)+ RNA was isolated with oligo d(T)- conjugated magnetic beads (Dynal- Invitrogen, Carlsbad, CA). 5 microgram of this RNA was used for construction of these libraries using the original SAGE protocol of Velculescu et al (Velculescu et al, 1995). Final concatenated ditags were cloned into Bluescript pKS (Stratagene, LA Jolla, CA) using BamHI site for blue white screening. Sequencing was perfomred at NIH Intramural Sequencing Centre (NISC). Low quality sequence data was eliminated by Phred analysis
|
| |
|
| Description |
not applicable
|
| Data processing |
eSAGE was used to extract tags and remove duplicate ditags from raw data.
|
| |
|
| Submission date |
Sep 02, 2006 |
| Last update date |
Nov 19, 2008 |
| Contact name |
saurabh joshi |
| E-mail(s) |
saurabhj@intra.niddk.nih.gov
|
| Phone |
301-594-0549
|
| Fax |
301-496-5239
|
| Organization name |
national institutes of health
|
| Department |
national institute of diabestes digestive and kidney diseases
|
| Lab |
laboratory of cellular and developmental biology
|
| Street address |
9000 rockville pk
|
| City |
bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL275 |
| Series (1) |
| GSE5802 |
Ovarian gene expression in the absence of FIGLA, an oocyte specific transcription factor |
|
