The non transformed pigmented melanocyte cell line PigMel was used at passage 37 for the SAGE library.
Growth protocol
Culture conditions of the PigMel cell line are described by Julé et al., in Pigment Cell Research 16:407-410, 2003.
Extracted molecule
polyA RNA
Extraction protocol
Dynabead mRNA direct kit
Description
Library was generated using the SAGE adaptation for downsized extracts (SADE) method (Virlon et al., P.N.A.S. 96:15286-15291, 1999). Cell lysates were centrifugated to discard melanin.
