Total RNA was isolated using mirVana miRNA Isolation Kit, with phenol by Life Technologies. RNA was eluted in 100 uL of the kit elution buffer.
Label
N/A
Label protocol
N/A
Hybridization protocol
N/A
Scan protocol
N/A
Data processing
Raw data was normalized by means of calculated normalization factors based on sum of positive controls and sum of microRNA counts per NanoString Technologies. MicroRNA with counts greater than background (average - 2 standard deviations) were considered detectable.