|
| Status |
Public on Jun 10, 2014 |
| Title |
T lymphoblastic tumor biopsy_diagnosis [TLBL_08] |
| Sample type |
RNA |
| |
|
| Source name |
lymphoblastic tumor biopsy_diagnosis
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: nodal tumor
diagnosis: lymphoblastic tumor biopsy
|
| Growth protocol |
Tissue or cells were lysated in Trizol Reagent (Invitrogen).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol Reagent (Invitrogen) following the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
100 ng of RNA were labeled using the Human miRNA Microarray kit (Agilent Technologies) following the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Hybridization mix was prepared using the Human miRNA Microarray kit (Agilent Technologies) following the manufacturer's instructions. Hybridization was performed at 55C for 20h. Washing was performed using the Gene Expression Wash Buffer kit (Agilent Technologies) following the manufacturer's instructions.
|
| Scan protocol |
Scanning was performed by the Agilent Microarray Scanner and the Agilent's Scan Control Software v. A.7.0.1 (Agilent Technologies) using the XDR feature and the recommended parameter settings.
|
| Description |
TLBL_08
|
| Data processing |
Raw image data were processed by the Feature Extraction software 9.5.1 (Agilent Technologies).
|
| |
|
| Submission date |
Mar 12, 2014 |
| Last update date |
Jun 10, 2014 |
| Contact name |
Riccardo Dalla-Favera |
| E-mail(s) |
rd10@cumc.columbia.edu
|
| Organization name |
Columbia University
|
| Street address |
1130 St Nicholas Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL8227 |
| Series (1) |
| GSE55846 |
miRNA profiling of pediatric T cell lymphoblastic lymphoma |
|
