|
| Status |
Public on Jun 21, 2014 |
| Title |
N-Thy001 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Frozen Tumor
|
| Organism |
Homo sapiens |
| Characteristics |
tumor: Thymic Carcinoma
Sex: M
who: TC
Stage: IVB
gtf2i: WT
|
| Treatment protocol |
none
|
| Growth protocol |
none
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
AllPrep DNA/RNA Mini Kit (Qiagen)
|
| Label |
Cy5
|
| Label protocol |
Genomic DNA ULS Labeling Kit (Agilent)
|
| |
|
| Channel 2 |
| Source name |
Promega
|
| Organism |
Homo sapiens |
| Characteristics |
reference: Commercial Normal Genomic DNA
Sex: Male
|
| Treatment protocol |
none
|
| Growth protocol |
none
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
AllPrep DNA/RNA Mini Kit (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
Genomic DNA ULS Labeling Kit (Agilent)
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner.
Images were quantified using Agilent Feature Extraction Software (version A.10.5).
|
| Description |
Array CGH of tumor DNA
|
| Data processing |
Agilent Feature Extraction Software (v 10.5) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Mar 13, 2014 |
| Last update date |
Jun 22, 2014 |
| Contact name |
Iacopo Petrini |
| E-mail(s) |
i.petrini@sssup.it
|
| Phone |
00393428566432
|
| Organization name |
Pisa University
|
| Street address |
Via Roma 67
|
| City |
Pisa |
| ZIP/Postal code |
56100 |
| Country |
Italy |
| |
|
| Platform ID |
GPL10123 |
| Series (1) |
| GSE55852 |
GTF2I Mutations are Common in Thymic Epithelial Tumors |
|
