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Sample GSM1350100 Query DataSets for GSM1350100
Status Public on Mar 01, 2015
Title BE2C_shGFP_rep1
Sample type RNA
 
Source name BE(2)-C/pLKO.1-shGFP 48h
Organism Homo sapiens
Characteristics cell line: BE(2)-C
treatment: control
Treatment protocol None
Growth protocol BE(2)-C cells were infected with pLKO.1-based lentiviruses expressing either shGFP or shMEIS2-43 for 48 hours
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the cell samples using Trizol (Invitrogen), and RNA quality and quantity was assessed using a Agilent BioAnalyzer and a Nanodrop Spectrophotometer.
Label biotin
Label protocol TdT-labled cRNA were prepared with an Affymetrix GeneChip WT Terminal labeling kit
 
Hybridization protocol Standard Affymetrix hybridization protocol
Scan protocol Standard Affymetrix scanning protocol
Description RNA expression value
Data processing Data were normalized, significance determined by ANOVA, and fold change calculated with the Partek Genomics Suite (Partek Inc., St. Louis, MO). Gene annotation enrichment analysis was performed with the Database for Annotation, Visualization and Integrated Discovery (DAVID) v6.7 for all significantly changed genes (≥ ±1.5 fold, P < 0.01) and Gene Set Enrichment Analysis (GSEA).
 
Submission date Mar 18, 2014
Last update date Mar 01, 2015
Contact name Justin Choi
E-mail(s) jeochoi@gmail.com
Phone 1-706-721-6757
Organization name Georgia Regents University
Department Cancer Center
Street address 1410 Laney Walker Blvd.
City Augusta
State/province GA
ZIP/Postal code 30912
Country USA
 
Platform ID GPL6244
Series (1)
GSE56003 Gene expression profiling of human neuroblastoma BE(2)-C cells with MEIS2 depletion

Data table header descriptions
ID_REF
VALUE quantile normalized gene level expression values

Data table
ID_REF VALUE
7896740 4.38911
7896742 9.70225
7896744 6.07621
7896754 7.90626
7896756 4.6644
7896759 6.85337
7896761 5.88782
7896779 6.28574
7896798 5.96546
7896817 5.52178
7896822 5.32766
7896859 6.0284
7896861 3.82202
7896863 4.82841
7896865 5.95737
7896878 6.76111
7896882 5.49049
7896908 7.77042
7896917 5.69582
7896921 6.07379

Total number of rows: 21949

Table truncated, full table size 340 Kbytes.




Supplementary file Size Download File type/resource
GSM1350100_shGFP_1.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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