NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1350103 Query DataSets for GSM1350103
Status Public on Mar 01, 2015
Title BE2C_shMEIS2_rep1
Sample type RNA
 
Source name BE(2)-C/pLKO.1-shMEIS2-43 48h
Organism Homo sapiens
Characteristics cell line: BE(2)-C
treatment: MEIS2 depletion
Treatment protocol None
Growth protocol BE(2)-C cells were infected with pLKO.1-based lentiviruses expressing either shGFP or shMEIS2-43 for 48 hours
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the cell samples using Trizol (Invitrogen), and RNA quality and quantity was assessed using a Agilent BioAnalyzer and a Nanodrop Spectrophotometer.
Label biotin
Label protocol TdT-labled cRNA were prepared with an Affymetrix GeneChip WT Terminal labeling kit
 
Hybridization protocol Standard Affymetrix hybridization protocol
Scan protocol Standard Affymetrix scanning protocol
Description RNA expression value
Data processing Data were normalized, significance determined by ANOVA, and fold change calculated with the Partek Genomics Suite (Partek Inc., St. Louis, MO). Gene annotation enrichment analysis was performed with the Database for Annotation, Visualization and Integrated Discovery (DAVID) v6.7 for all significantly changed genes (≥ ±1.5 fold, P < 0.01) and Gene Set Enrichment Analysis (GSEA).
 
Submission date Mar 18, 2014
Last update date Mar 01, 2015
Contact name Justin Choi
E-mail(s) jeochoi@gmail.com
Phone 1-706-721-6757
Organization name Georgia Regents University
Department Cancer Center
Street address 1410 Laney Walker Blvd.
City Augusta
State/province GA
ZIP/Postal code 30912
Country USA
 
Platform ID GPL6244
Series (1)
GSE56003 Gene expression profiling of human neuroblastoma BE(2)-C cells with MEIS2 depletion

Data table header descriptions
ID_REF
VALUE quantile normalized gene level expression values

Data table
ID_REF VALUE
7896740 4.5382
7896742 9.79551
7896744 6.87049
7896754 8.54748
7896756 4.66826
7896759 7.05442
7896761 5.97636
7896779 6.35836
7896798 5.85606
7896817 5.47245
7896822 5.39065
7896859 6.00328
7896861 3.9387
7896863 4.8863
7896865 5.85772
7896878 6.63828
7896882 5.43621
7896908 7.45266
7896917 5.56158
7896921 6.30134

Total number of rows: 21949

Table truncated, full table size 340 Kbytes.




Supplementary file Size Download File type/resource
GSM1350103_shMEIS2-43_1.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.