|
Status |
Public on Sep 08, 2006 |
Title |
Anti-H3 ChIP-chip in Leu3-overexpressing strain ckl5128_LEU3antiH3_2 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
anti-H3 ChIP
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
Yeast strain: BY4720-LEU3null expressing vector: pRS416-TEF1-MBPLeu3R643G, which overexpresses constitutively active Leu3. anti-H3 ChIPed DNA
|
Biomaterial provider |
Cheol-Koo Lee in Lieb lab UNC
|
Treatment protocol |
anti-H3 ChIPed DNA
|
Growth protocol |
cells were grown to OD of 1
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Fixed cells were washed and lysed using bead beater and sonicated to get the extract
|
Label |
Cy3
|
|
|
Channel 2 |
Source name |
input extract
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
Yeast strain: BY4720-LEU3null expressing vector: pRS416-TEF1-MBPLeu3R643G, which overexpresses constitutively active Leu3. supernatant DNA as reference DNA
|
Biomaterial provider |
Cheol-Koo Lee in Lieb lab UNC
|
Treatment protocol |
supernatant DNA as reference DNA
|
Growth protocol |
cells were grown to OD of 1
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Fixed cells were washed and lysed using bead beater and sonicated to get the extract
|
Label |
Cy5
|
|
|
|
Scan protocol |
Fluorescent array images were collected for both Cy3 and Cy5 with a GenePix 4000A fluorescent scanner and image intensity data were extracted and analyzed with GenePix Pro 5.0 analysis
|
Description |
Yeast strain: BY4720-LEU3null expressing vector: pRS416-TEF1-MBPLeu3R643G, which overexpresses constitutively active Leu3. anti-Histone3 chiped DNA is Cy3-labeled Flagged spots(low image quality) have been filtered.
|
Data processing |
lowess normalization
|
|
|
Submission date |
Sep 07, 2006 |
Last update date |
Oct 13, 2006 |
Contact name |
Jason D Lieb |
E-mail(s) |
jlieb@bio.unc.edu
|
Phone |
(919) 843-3228
|
Fax |
(919) 962-1625
|
URL |
http://www.bio.unc.edu/faculty/lieb/labpages/home.shtml
|
Organization name |
University of North Carolina at Chapel Hill
|
Department |
Department of Biology
|
Street address |
CB# 3280, 202 Fordham Hall
|
City |
Chapel Hill |
State/province |
NC |
ZIP/Postal code |
27599-3280 |
Country |
USA |
|
|
Platform ID |
GPL4279 |
Series (2) |
GSE5782 |
anti-H3 Chip-chip in Leu3overexpress or Leu3AD starins |
GSE5785 |
Comparison of Leu3 binding in vitro and in vivo |
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