|
| Status |
Public on Feb 08, 2007 |
| Title |
Celegans_gacA_8hours_RepA |
| Sample type |
RNA |
| |
|
| Source name |
Celegans_gacA_8hours
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
Strain: fer-15(b26);fem-1(hc17)
Age: young adult
Tissue: whole animal
|
| Treatment protocol |
Animals were transferred to P. aeruginosa PA14 mutant strain gacA for 8 hours prior to harvest.
|
| Growth protocol |
fer-15;fem-1 animals were synchronized by hypochlorite treatment and first larval stage (L1) arrest. Arrested L1s were placed onto NGM plates seeded with OP50 and grown at the restrictive temperature (25°C) in order to obtain sterile adults. After ~52 hours of growth at 25°C (young adulthood), animals were transferred to SK plates seeded either with OP50, wild-type PA14 or gacA mutant PA14. Animals were harvested at 4 hours and 8 hours after transfer.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRI Reagent (Molecular Research Center)
|
| Label |
biotin
|
| Label protocol |
Affymetrix
|
| |
|
| Hybridization protocol |
Affymetrix
|
| Scan protocol |
Affymetrix
|
| Description |
fer-15;fem-1 animals were synchronized by hypochlorite treatment and first larval stage (L1) arrest. Arrested L1s were placed onto NGM plates seeded with OP50 and grown at the restrictive temperature (25°C) in order to obtain sterile adults. After ~52 hours of growth at 25°C (young adulthood), animals were transferred to SK plates seeded either with OP50, wild-type PA14 or gacA mutant PA14. Animals were harvested at 4 hours and 8 hours after transfer.
|
| Data processing |
Resolver Gene Expression Data Analysis System, version 5.1 (Rosetta Inpharmatics)
|
| |
|
| Submission date |
Sep 07, 2006 |
| Last update date |
Jun 23, 2008 |
| Contact name |
Emily Troemel |
| E-mail(s) |
etroemel@ucsd.edu
|
| Organization name |
University of California, San Diego
|
| Department |
Cell and Developmental Biology
|
| Lab |
Troemel
|
| Street address |
9500 Gilman Dr, #0349
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE5793 |
C. elegans gene expression in response to the pathogenic P. aeruginosa strain PA14. |
|
