|
| Status |
Public on Sep 12, 2006 |
| Title |
ChIP of LEU3p-DB 6/14/03 No.2 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
input extract
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Yeast strain: BY4720-LEU3null
expressing vector: pRS416-TEF1-MBPLeu3pDB, which overexpresses DNA-binding domain of Leu3(1-147aa).
supernatant DNA as reference DNA
|
| Biomaterial provider |
Neil Clarke Lab in Johns Hopkins Medical School
|
| Treatment protocol |
supernatant DNA as reference DNA
|
| Growth protocol |
cells were grown to OD of 1
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Fixed cells were washed and lysed using bead beater and sonicated to get the extract
|
| Label |
Cy3
|
| |
|
| Channel 2 |
| Source name |
anti-MBP-Leu3DB ChIP
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Yeast strain: BY4720-LEU3null
expressing vector: pRS416-TEF1-MBPLeu3pDB, which overexpresses DNA-binding domain of Leu3(1-147aa).
anti-MBP-Leu3DB ChIPed DNA
|
| Biomaterial provider |
Neil Clarke Lab in Johns Hopkins Medical School
|
| Treatment protocol |
anti-MBP-Leu3DB ChIP
|
| Growth protocol |
cells were grown to OD of 1
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Fixed cells were washed and lysed using bead beater and sonicated to get the extract
|
| Label |
Cy5
|
| |
|
| |
| Scan protocol |
Fluorescent array images were collected for both Cy3 and Cy5 with a GenePix 4000A fluorescent scanner and image intensity data were extracted and analyzed with GenePix Pro 5.0 analysis
|
| Description |
overexpressing LEU3p DNA-binding domain (1-147aa) under TDH3 promoter in pRS416-TEF1-MBPLeu3pAD(DB-null-BspEI) vector.
yeast strain: BY4720, leu3null
MBP-tagged Leu3DB
anti-MBP(Abcam65)
done in 6/14/2003 #2
Flagged spots(low image quality) have been filtered.
|
| Data processing |
lowess normalization
|
| |
|
| Submission date |
Sep 08, 2006 |
| Last update date |
Oct 13, 2006 |
| Contact name |
Jason D Lieb |
| E-mail(s) |
jlieb@bio.unc.edu
|
| Phone |
(919) 843-3228
|
| Fax |
(919) 962-1625
|
| URL |
http://www.bio.unc.edu/faculty/lieb/labpages/home.shtml
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Department of Biology
|
| Street address |
CB# 3280, 202 Fordham Hall
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599-3280 |
| Country |
USA |
| |
|
| Platform ID |
GPL4278 |
| Series (2) |
| GSE5785 |
Comparison of Leu3 binding in vitro and in vivo |
| GSE6016 |
DIP-chip: Rapid and accurate determination of DNA-binding specificity |
|
