|
Status |
Public on Sep 12, 2006 |
Title |
DIP of MBP as negative control No.5. Cy3 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
4 nM MBP DIP
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
in vitro amylose resin precipitaion of misture of MBP/naked yeast genome as negative control of MBP::Leu3DB DIP-chip [MBP] = 4 nM
|
Biomaterial provider |
Neil Clarke Lab in Johns Hopkins Medical School
|
Treatment protocol |
4 nM MBP DNA-ImmunoPrecipitation
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA is extracted and then sonicated
|
Label |
Cy3
|
|
|
Channel 2 |
Source name |
input DNA
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
naked yeast genomic DNA
|
Biomaterial provider |
Neil Clarke Lab in Johns Hopkins Medical School
|
Growth protocol |
Genomic DNA is extracted and then sonicated
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA is extracted and then sonicated
|
Label |
Cy5
|
|
|
|
Scan protocol |
Fluorescent array images were collected for both Cy3 and Cy5 with a GenePix 4000A fluorescent scanner and image intensity data were extracted and analyzed with GenePix Pro 5.0 analysis
|
Description |
in vitro amylose resin precipitaion of misture of MBP/naked yeast genome as negative control of MBP::Leu3DB DIP-chip [MBP] = 4 nM done in Nov./2003 #5 *IPed DNA is Cy3-labelled. Flagged spots(low image quality) have been filtered.
|
Data processing |
lowess normalization
|
|
|
Submission date |
Sep 08, 2006 |
Last update date |
Oct 13, 2006 |
Contact name |
Jason D Lieb |
E-mail(s) |
jlieb@bio.unc.edu
|
Phone |
(919) 843-3228
|
Fax |
(919) 962-1625
|
URL |
http://www.bio.unc.edu/faculty/lieb/labpages/home.shtml
|
Organization name |
University of North Carolina at Chapel Hill
|
Department |
Department of Biology
|
Street address |
CB# 3280, 202 Fordham Hall
|
City |
Chapel Hill |
State/province |
NC |
ZIP/Postal code |
27599-3280 |
Country |
USA |
|
|
Platform ID |
GPL4278 |
Series (2) |
GSE5785 |
Comparison of Leu3 binding in vitro and in vivo |
GSE6016 |
DIP-chip: Rapid and accurate determination of DNA-binding specificity |
|