|
| Status |
Public on Mar 14, 2009 |
| Title |
Human_TK25T_clinically isolated tissue |
| Sample type |
RNA |
| |
|
| Source name |
human breast cancer
|
| Organism |
Homo sapiens |
| Characteristics |
clinical isolate, flash-frozen
|
| Treatment protocol |
Tissues were surgically isolated and were immediately frozen in liquid nitrogen. Tissues were lysed and total RNA was extracted using the Sepasol-I (WAKO)
|
| Growth protocol |
not received any pre-operative adjuvant hormone or chemotherapy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit
|
| Label |
biotin
|
| Label protocol |
Enzo Biotin labeling
|
| |
|
| Hybridization protocol |
According to standard Affymetrix protocol. 15ug of cRNA was fragmented, fragmented product was checked on gel(average size 100nt), Hybridization cocktail added and hybed on HG_U133A arrays. Washed with non-stringent wash buffer (A) and stained with SAPE solution.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from embryos younger than nuclear cycle 9, i.e. before zygotic genome activation.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Sep 11, 2006 |
| Last update date |
Mar 16, 2009 |
| Contact name |
shinobu Masuda |
| E-mail(s) |
masuda.shinobu@nihon-u.ac.jp
|
| Phone |
81-3-3972-8111
|
| Organization name |
Nihon Univeristy School of Medicine
|
| Department |
pathology
|
| Street address |
30-1 Ohyaguchikami-cho
|
| City |
Itabashi |
| State/province |
Tokyo |
| ZIP/Postal code |
173-8610 |
| Country |
Japan |
| |
|
| Platform ID |
GPL8300 |
| Series (1) |
| GSE6367 |
Gene profile of breast cancers with immunohistochemical phenotypes of ER+/- and/or HER2+/- |
|
