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Sample GSM1356642 Query DataSets for GSM1356642
Status Public on Mar 25, 2014
Title LCA4
Sample type RNA
Source name left carotid artery, saline-treated control, 7 days ligated
Organism Mus musculus
Characteristics treatment: saline-treated control, 7 days ligated
strain: C57Bl6
age: 9 weeks
tissue: carotid artery intima
Treatment protocol Mice were treated with either saline control or 5-Aza-2'deoxycytidine (0.2mg/kg/day) by daily I.P. injection for 3 weeks. After 2 weeks of treament, 3 out of the 4 caudal branches of the left carotid artery (LCA) of mice were ligated while the right carotid artery (RCA) was left untouched as the contralateral internal control. After 1 week of ligation, mice were sacrificed by CO2 inhalation.
Growth protocol C57Bl6 mice were fed normal chow diet for 9 weeks.
Extracted molecule total RNA
Extraction protocol Mice were perfused with saline and carotid arteries were extracted and flushed with 150 ul of Qiazol. The Qiagen miReasy Kit was used to extract and purify total RNA. Quality control was performed with Agilent Bioanalyzer.
Label biotin
Label protocol PCR product was prepared with standard Illumina protocol for arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol BeadChips were scanned using Illumina HiScan.
Description endothelial-enriched RNA from carotid artery of C57Bl6 male 9 weeks of age
endothelial-enriched RNA (3 pooled arteries),mouse left carotid artery, 7 days ligated, saline-treated control, replicate #3
Data processing The data were normalized using quantile-normalization with GenomeStudio V2010.3
Submission date Mar 24, 2014
Last update date Mar 25, 2014
Contact name Jessilyn Dunn
Phone 404-727-3223
Organization name Georgia Tech & Emory University
Department Biomedical Engineering
Lab Hanjoong Jp
Street address 1760 Haygood Drive HSRB E197
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
Platform ID GPL6887
Series (1)
GSE56143 Identification of mechano- and/or 5Aza-sensitive genes in mouse carotid artery endothelium in vivo exposed to disturbed blood flow for 7 days

Data table header descriptions
VALUE The values are linear and represent quantile-normalized expression signal
LCA4.Detection Pval

Data table
ID_REF VALUE LCA4.Detection Pval
ILMN_2417611 78.44438 0.8076923
ILMN_2762289 78.09851 0.8376068
ILMN_2896528 210.3806 0
ILMN_2721178 124.5566 0.002136752
ILMN_2458837 78.36747 0.8119658
ILMN_3033922 211.9761 0
ILMN_3092673 526.5319 0
ILMN_1230777 439.3993 0
ILMN_1246069 90.43201 0.01602564
ILMN_1232042 77.56645 0.8878205
ILMN_1243193 163.698 0
ILMN_2524361 84.15923 0.1474359
ILMN_1242440 85.47591 0.08440171
ILMN_1233188 108.6331 0.002136752
ILMN_2543688 366.3447 0
ILMN_1259789 96.16778 0.00534188
ILMN_2816356 83.61313 0.1816239
ILMN_1224596 82.53159 0.2863248
ILMN_1233643 88.43039 0.02457265
ILMN_2808939 180.4719 0

Total number of rows: 45281

Table truncated, full table size 1346 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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