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Sample GSM1356643 Query DataSets for GSM1356643
Status Public on Mar 25, 2014
Title RCA1
Sample type RNA
Source name right carotid artery, saline-treated control, unligated contralateral control
Organism Mus musculus
Characteristics treatment: saline-treated control, unligated contralateral control
strain: C57Bl6
age: 9 weeks
tissue: carotid artery intima
Treatment protocol Mice were treated with either saline control or 5-Aza-2'deoxycytidine (0.2mg/kg/day) by daily I.P. injection for 3 weeks. After 2 weeks of treament, 3 out of the 4 caudal branches of the left carotid artery (LCA) of mice were ligated while the right carotid artery (RCA) was left untouched as the contralateral internal control. After 1 week of ligation, mice were sacrificed by CO2 inhalation.
Growth protocol C57Bl6 mice were fed normal chow diet for 9 weeks.
Extracted molecule total RNA
Extraction protocol Mice were perfused with saline and carotid arteries were extracted and flushed with 150 ul of Qiazol. The Qiagen miReasy Kit was used to extract and purify total RNA. Quality control was performed with Agilent Bioanalyzer.
Label biotin
Label protocol PCR product was prepared with standard Illumina protocol for arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol BeadChips were scanned using Illumina HiScan.
Description endothelial-enriched RNA from carotid artery of C57Bl6 male 9 weeks of age
endothelial-enriched RNA (3 pooled arteries),mouse right carotid artery, unligated contralateral control, saline-treated control, replicate #1
Data processing The data were normalized using quantile-normalization with GenomeStudio V2010.3
Submission date Mar 24, 2014
Last update date Mar 25, 2014
Contact name Jessilyn Dunn
Phone 404-727-3223
Organization name Georgia Tech & Emory University
Department Biomedical Engineering
Lab Hanjoong Jp
Street address 1760 Haygood Drive HSRB E197
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
Platform ID GPL6887
Series (1)
GSE56143 Identification of mechano- and/or 5Aza-sensitive genes in mouse carotid artery endothelium in vivo exposed to disturbed blood flow for 7 days

Data table header descriptions
VALUE The values are linear and represent quantile-normalized expression signal
RCA1.Detection Pval

Data table
ID_REF VALUE RCA1.Detection Pval
ILMN_2417611 81.04424 0.4797009
ILMN_2762289 83.33553 0.2094017
ILMN_2896528 210.6147 0
ILMN_2721178 125.1888 0.001068376
ILMN_2458837 81.64714 0.3985043
ILMN_3033922 340.1896 0
ILMN_3092673 611.872 0
ILMN_1230777 500.1032 0
ILMN_1246069 90.36004 0.01602564
ILMN_1232042 82.66283 0.2852564
ILMN_1243193 184.2108 0.001068376
ILMN_2524361 88.30182 0.02991453
ILMN_1242440 79.32291 0.7051282
ILMN_1233188 127.2474 0.001068376
ILMN_2543688 304.5765 0
ILMN_1259789 89.24905 0.0224359
ILMN_2816356 84.40915 0.1452992
ILMN_1224596 84.74565 0.1346154
ILMN_1233643 88.80823 0.02564103
ILMN_2808939 137.3289 0.001068376

Total number of rows: 45281

Table truncated, full table size 1390 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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