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Sample GSM1356644 Query DataSets for GSM1356644
Status Public on Mar 25, 2014
Title RCA2
Sample type RNA
Source name right carotid artery, saline-treated control, unligated contralateral control
Organism Mus musculus
Characteristics treatment: saline-treated control, unligated contralateral control
strain: C57Bl6
age: 9 weeks
tissue: carotid artery intima
Treatment protocol Mice were treated with either saline control or 5-Aza-2'deoxycytidine (0.2mg/kg/day) by daily I.P. injection for 3 weeks. After 2 weeks of treament, 3 out of the 4 caudal branches of the left carotid artery (LCA) of mice were ligated while the right carotid artery (RCA) was left untouched as the contralateral internal control. After 1 week of ligation, mice were sacrificed by CO2 inhalation.
Growth protocol C57Bl6 mice were fed normal chow diet for 9 weeks.
Extracted molecule total RNA
Extraction protocol Mice were perfused with saline and carotid arteries were extracted and flushed with 150 ul of Qiazol. The Qiagen miReasy Kit was used to extract and purify total RNA. Quality control was performed with Agilent Bioanalyzer.
Label biotin
Label protocol PCR product was prepared with standard Illumina protocol for arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol BeadChips were scanned using Illumina HiScan.
Description endothelial-enriched RNA from carotid artery of C57Bl6 male 9 weeks of age
endothelial-enriched RNA (3 pooled arteries),mouse right carotid artery, unligated contralateral control, saline-treated control, replicate #2
Data processing The data were normalized using quantile-normalization with GenomeStudio V2010.3
Submission date Mar 24, 2014
Last update date Mar 25, 2014
Contact name Jessilyn Dunn
Phone 404-727-3223
Organization name Georgia Tech & Emory University
Department Biomedical Engineering
Lab Hanjoong Jp
Street address 1760 Haygood Drive HSRB E197
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
Platform ID GPL6887
Series (1)
GSE56143 Identification of mechano- and/or 5Aza-sensitive genes in mouse carotid artery endothelium in vivo exposed to disturbed blood flow for 7 days

Data table header descriptions
VALUE The values are linear and represent quantile-normalized expression signal
RCA2.Detection Pval

Data table
ID_REF VALUE RCA2.Detection Pval
ILMN_2417611 80.69614 0.5502137
ILMN_2762289 79.6775 0.6688034
ILMN_2896528 209.5319 0
ILMN_2721178 119.425 0.001068376
ILMN_2458837 80.69121 0.5502137
ILMN_3033922 365.5222 0
ILMN_3092673 711.4505 0
ILMN_1230777 545.1741 0
ILMN_1246069 93.50182 0.007478632
ILMN_1232042 80.35401 0.590812
ILMN_1243193 193.9017 0
ILMN_2524361 85.43633 0.1303419
ILMN_1242440 80.49428 0.573718
ILMN_1233188 121.7459 0.001068376
ILMN_2543688 239.9856 0
ILMN_1259789 84.06487 0.2104701
ILMN_2816356 92.30865 0.01068376
ILMN_1224596 88.38426 0.04059829
ILMN_1233643 98.38156 0.00534188
ILMN_2808939 149.7787 0

Total number of rows: 45281

Table truncated, full table size 1342 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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