|
| Status |
Public on Mar 25, 2014 |
| Title |
AzaLCA2 |
| Sample type |
RNA |
| |
|
| Source name |
left carotid artery, 21 days of 5-Aza-2'deoxycytidine treatment (0.2mg/kg/day by I.P. injection), 7 days ligated
|
| Organism |
Mus musculus |
| Characteristics |
treatment: 5-Aza-2'deoxycytidine treatment, 7 days ligated
strain: C57Bl6
age: 9 weeks
tissue: carotid artery intima
|
| Treatment protocol |
Mice were treated with either saline control or 5-Aza-2'deoxycytidine (0.2mg/kg/day) by daily I.P. injection for 3 weeks. After 2 weeks of treament, 3 out of the 4 caudal branches of the left carotid artery (LCA) of mice were ligated while the right carotid artery (RCA) was left untouched as the contralateral internal control. After 1 week of ligation, mice were sacrificed by CO2 inhalation.
|
| Growth protocol |
C57Bl6 mice were fed normal chow diet for 9 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mice were perfused with saline and carotid arteries were extracted and flushed with 150 ul of Qiazol. The Qiagen miReasy Kit was used to extract and purify total RNA. Quality control was performed with Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
PCR product was prepared with standard Illumina protocol for arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
BeadChips were scanned using Illumina HiScan.
|
| Description |
endothelial-enriched RNA from carotid artery of C57Bl6 male 9 weeks of age
endothelial-enriched RNA (3 pooled arteries),mouse left carotid artery, 7 days ligated, 21 days of 5-Aza-2'deoxycytidine treatment (0.2mg/kg/day by I.P. injection), replicate #2
|
| Data processing |
The data were normalized using quantile-normalization with GenomeStudio V2010.3
|
| |
|
| Submission date |
Mar 24, 2014 |
| Last update date |
Mar 25, 2014 |
| Contact name |
Jessilyn Dunn |
| E-mail(s) |
jpdunn@emory.edu
|
| Phone |
404-727-3223
|
| Organization name |
Georgia Tech & Emory University
|
| Department |
Biomedical Engineering
|
| Lab |
Hanjoong Jp
|
| Street address |
1760 Haygood Drive HSRB E197
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE56143 |
Identification of mechano- and/or 5Aza-sensitive genes in mouse carotid artery endothelium in vivo exposed to disturbed blood flow for 7 days |
|
