|
| Status |
Public on May 01, 2014 |
| Title |
72h_A_X1(2) |
| Sample type |
RNA |
| |
|
| Source name |
X1 neoblasts, anterior-facing wounds, 72h regeneration, replicate 2
|
| Organism |
Schmidtea mediterranea |
| Characteristics |
strain: CIW4
cell type: neoblasts
regeneration type: head
|
| Treatment protocol |
Tissues ~200 microns from amputation sites were diced and mascerated in 1xCMF (calcium-magnesium free media) with collagenase, labeled with Hoechst dye and propidium iodide, and FACS sorted as the population of G2/S/M cells of high DNA content
|
| Growth protocol |
Asexual planarians, strain CIW4 Schmidtea mediterranea, were cultured at 19 degrees in 1x planarian water after amputation of heads and tails.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted in trizol followed by purification of the aqueous phase using RNAeasy kits. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer
|
| Label |
Cy5
|
| Label protocol |
WTA2 whole genome amplification (Sigma) followed by Kreatech labeling with Cy5
|
| |
|
| Hybridization protocol |
Hybridizations were performed according to manufacturerer's protocol
|
| Scan protocol |
Scan was performed according to manufacturer's protocol
|
| Description |
Gene expression in neoblasts after 72h head regeneration
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters (protocol GE1-v1_91 and Grid: 012391_D_20060331) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Mar 25, 2014 |
| Last update date |
May 01, 2014 |
| Contact name |
Christian Paul Petersen |
| E-mail(s) |
christian-p-petersen@northwestern.edu
|
| Phone |
847-467-3553
|
| Organization name |
Northwestern University
|
| Department |
Molecular Biosciences
|
| Street address |
2205 Tech Drive, Hogan 2-100
|
| City |
Evanston |
| State/province |
IL |
| ZIP/Postal code |
60201 |
| Country |
USA |
| |
|
| Platform ID |
GPL18464 |
| Series (1) |
| GSE56178 |
Neoblast expression in head and tail regeneration |
|
