|
| Status |
Public on Jun 09, 2014 |
| Title |
prg-1_L3_mRNA |
| Sample type |
SRA |
| |
|
| Source name |
prg-1 L3, whole worms
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
tissue: whole worm
genotype: prg-1 mutant
strain: wm161
|
| Growth protocol |
Animals were grown at 20˚C with feeding on OP50
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted from each of the six different developmental stages using the Trizol protocol. Small RNAs were size-selected by gel electrophoresis, and used poly(A) to extract L1-L4 mRNA.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Reads were aligned to the ce10 genome assembly using TopHat v2.0.8
For each sample, FPKM was computed by Cufflinks v2.1.1
Genome_build: ce10
Supplementary_files_format_and_content: gene_fpkm
|
| |
|
| Submission date |
Mar 27, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Jiajia Wang |
| E-mail(s) |
wangjiajia_816@126.com
|
| Organization name |
Institute of Zoology, Chinese Academy of Sciences
|
| Lab |
Key Laboratory of the Zoological Systematics and Evolution
|
| Street address |
1 Beichen West Road, Chaoyang District
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platform ID |
GPL13657 |
| Series (2) |
| GSE56271 |
The expression mRNAs after prg-1 mutation |
| GSE56274 |
The expression of mRNAs and small RNAs after prg-1 mutation |
|
| Relations |
| BioSample |
SAMN02709116 |
| SRA |
SRX501866 |
