|
Status |
Public on Apr 01, 2014 |
Title |
PCBM1641, Passage 5, Technical Replicate 3 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
PCBM1641_MSCs_passage 5
|
Organism |
Homo sapiens |
Characteristics |
donor id: PCBM1641 gender: Male cell type: human multipotent stromal cells (MSCs) derived from bone marrow passage: passage 5
|
Treatment protocol |
MSCs were passaged after reaching 80% confluency as determined by one sole cell biologist
|
Growth protocol |
MSCs were grown in alpha minimum essential media with 10% FBS, 1% L-gluatmine & 1% penicillin G and streptomycin under humidified atmosphere of 5% CO2 at 37C
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted by the Qiagen Rneasy Mini kit
|
Label |
Hyper5
|
Label protocol |
Individual RNA material was labeled with Hyper5 and reference material (P3star or P3*) was labeled with Cy3. See manuscript for more detailed description
|
|
|
Channel 2 |
Source name |
pooled reference (P3star)
|
Organism |
Homo sapiens |
Characteristics |
sample type: Pooled reference Sample rna source: equal portions of total RNA from 6 donors (PCBM1641 P3, PCBM1632 P3, PCBM1662 P3, 8F3560 P3, 167696 P3, 110877 P3).
|
Treatment protocol |
MSCs were passaged after reaching 80% confluency as determined by one sole cell biologist
|
Growth protocol |
MSCs were grown in alpha minimum essential media with 10% FBS, 1% L-gluatmine & 1% penicillin G and streptomycin under humidified atmosphere of 5% CO2 at 37C
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted by the Qiagen Rneasy Mini kit
|
Label |
Cy3
|
Label protocol |
Individual RNA material was labeled with Hyper5 and reference material (P3star or P3*) was labeled with Cy3. See manuscript for more detailed description
|
|
|
|
Hybridization protocol |
Hyper5 and Cy3 labeled samples were mixed and hybridized on he Maui hybridization system for 16 hours at 42C
|
Scan protocol |
Microarray slides were scanned using the 4000B GenePix Axon Scanner. PMT voltages of both channels were adjusted such that their intensity histograms overlap.
|
Description |
{PCBM1641_P5_3} Cy5/Cy3 [PCBM1641|P3star]
|
Data processing |
LOESS/Linear normalization was performed using ArrayTrack software (FDA) with no background subtraction
|
|
|
Submission date |
Mar 31, 2014 |
Last update date |
Oct 23, 2015 |
Contact name |
Raj K Puri |
E-mail(s) |
raj.puri@fda.hhs.gov
|
Phone |
240-402-9171
|
Organization name |
FDA
|
Street address |
10903 New Hampshire Ave
|
City |
Silver Spring |
State/province |
Maryland |
ZIP/Postal code |
20993 |
Country |
USA |
|
|
Platform ID |
GPL18503 |
Series (2) |
GSE56362 |
Human Multipotent Stromal Cells: Passage 3 vs. Passage 5 vs. Passage 7 |
GSE74303 |
Identification of Predictive Gene Markers for Multipotent Stromal Cell Proliferation |
|