|
| Status |
Public on Oct 01, 2015 |
| Title |
SJW/Sal non-tumor 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Non-tumor area of mouse small intestine with SJW diet and saline injection
|
| Organism |
Mus musculus |
| Characteristics |
treatment: SJW diet and saline injection
gender: Male
strain: C57BL/6
tissue: Non-tumor area of mouse small intestine
|
| Treatment protocol |
Collected tissues from fresh small intestines
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol from liver and purified with QIAGEN kit (#74104) following manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
cDNA was generated with SuperScript® Indirect cDNA Labeling Core kit (Invitrogen) and labeled with Cy3/Cy5-mono reactive dye pack (GE healthcare), and the labeled cDNA was purified with Qiagen kit (#28004)
|
| |
|
| Channel 2 |
| Source name |
Ambion Mouse Assorted Total RNA
|
| Organism |
Mus musculus |
| Characteristics |
strain: Swiss Webster
tissue: brain, embryo (10–12 days), heart, kidney, liver, lung, ovary, spleen, testicle, and thymus
|
| Treatment protocol |
Collected tissues from fresh small intestines
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol from liver and purified with QIAGEN kit (#74104) following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
cDNA was generated with SuperScript® Indirect cDNA Labeling Core kit (Invitrogen) and labeled with Cy3/Cy5-mono reactive dye pack (GE healthcare), and the labeled cDNA was purified with Qiagen kit (#28004)
|
| |
|
| |
| Hybridization protocol |
Blocking buffer and Hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner.
|
| Data processing |
mages were quantified using Agilent Feature Extraction Software. Agilent Feature Extraction Software was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Apr 07, 2014 |
| Last update date |
Oct 01, 2015 |
| Contact name |
yan cai |
| E-mail(s) |
yan.cai@nih.gov
|
| Phone |
3014020062
|
| Organization name |
NIH
|
| Department |
NCI
|
| Lab |
LM
|
| Street address |
9000 rockville pike
|
| City |
bethesda |
| State/province |
maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE56571 |
Gene expression of mouse intestine treated with azoxymethane and/or St. John's wort |
|
