|
Status |
Public on Oct 01, 2015 |
Title |
SJW/Sal non-tumor 2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Non-tumor area of mouse small intestine with SJW diet and saline injection
|
Organism |
Mus musculus |
Characteristics |
treatment: SJW diet and saline injection gender: Male strain: C57BL/6 tissue: Non-tumor area of mouse small intestine
|
Treatment protocol |
Collected tissues from fresh small intestines
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol from liver and purified with QIAGEN kit (#74104) following manufacturer's instructions.
|
Label |
Cy5
|
Label protocol |
cDNA was generated with SuperScript® Indirect cDNA Labeling Core kit (Invitrogen) and labeled with Cy3/Cy5-mono reactive dye pack (GE healthcare), and the labeled cDNA was purified with Qiagen kit (#28004)
|
|
|
Channel 2 |
Source name |
Ambion Mouse Assorted Total RNA
|
Organism |
Mus musculus |
Characteristics |
strain: Swiss Webster tissue: brain, embryo (10–12 days), heart, kidney, liver, lung, ovary, spleen, testicle, and thymus
|
Treatment protocol |
Collected tissues from fresh small intestines
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol from liver and purified with QIAGEN kit (#74104) following manufacturer's instructions.
|
Label |
Cy3
|
Label protocol |
cDNA was generated with SuperScript® Indirect cDNA Labeling Core kit (Invitrogen) and labeled with Cy3/Cy5-mono reactive dye pack (GE healthcare), and the labeled cDNA was purified with Qiagen kit (#28004)
|
|
|
|
Hybridization protocol |
Blocking buffer and Hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
Scan protocol |
Scanned on an Agilent G2565AA scanner.
|
Data processing |
mages were quantified using Agilent Feature Extraction Software. Agilent Feature Extraction Software was used for background subtraction and LOWESS normalization.
|
|
|
Submission date |
Apr 07, 2014 |
Last update date |
Oct 01, 2015 |
Contact name |
yan cai |
E-mail(s) |
yan.cai@nih.gov
|
Phone |
3014020062
|
Organization name |
NIH
|
Department |
NCI
|
Lab |
LM
|
Street address |
9000 rockville pike
|
City |
bethesda |
State/province |
maryland |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL7202 |
Series (1) |
GSE56571 |
Gene expression of mouse intestine treated with azoxymethane and/or St. John's wort |
|