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Sample GSM1366774 Query DataSets for GSM1366774
Status Public on Apr 11, 2014
Title kidney_1
Sample type SRA
 
Source name Kidney
Organism Esox lucius
Characteristics age: juvenile
tissue: Kidney
age: male
genotype: wild type
Extracted molecule total RNA
Extraction protocol All tissues for RNA-seq were extracted from a single, juvenile, male pike caught November 1, 2011 in the Portage Diversion/Assiniboine River Floodway near Portage la Prairie, MB, Canada (N50.00747 W98.38181). The animal was euthanized and then frozen on dry ice until sampling, and subsequently at -80°C. Tissues were extracted by mixer-mill (Retsch) homogenization in Trizol (Invitrogen), followed by column clean-up using the RNeasy kit (QIAGEN).
RNA libraries were prepared for sequencing using standard Illumina protocols.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Description Kidney
Data processing Please refer to TSA: GATF00000000.1 (http://www.ncbi.nlm.nih.gov/nuccore/601035834)
Fragments Per Kilobase of exon per Megabase of library size (FPKM) were calculated using CLC Genomics Workbench v6.5
Genome_build: GATF00000000.1
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample ...
 
Submission date Apr 10, 2014
Last update date May 15, 2019
Contact name Ben F Koop
E-mail(s) bkoop@uvic.ca
Phone (250) 472-4067
Organization name The University of Victoria
Department Biology
Lab Centre for Biomedical Research
Street address PO Box 3020 STN CSC
City Victoria
State/province BC
ZIP/Postal code V8W 3N5
Country Canada
 
Platform ID GPL18557
Series (1)
GSE56679 Esox lucius transcriptome
Relations
BioSample SAMN02724948
SRA SRX514263

Supplementary file Size Download File type/resource
GSM1366774_eluc_kidney_1.txt.gz 225.4 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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