 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 11, 2014 |
| Title |
spleen_1 |
| Sample type |
SRA |
| |
|
| Source name |
Spleen
|
| Organism |
Esox lucius |
| Characteristics |
age: juvenile
tissue: Spleen
age: male
genotype: wild type
|
| Extracted molecule |
total RNA |
| Extraction protocol |
All tissues for RNA-seq were extracted from a single, juvenile, male pike caught November 1, 2011 in the Portage Diversion/Assiniboine River Floodway near Portage la Prairie, MB, Canada (N50.00747 W98.38181). The animal was euthanized and then frozen on dry ice until sampling, and subsequently at -80°C. Tissues were extracted by mixer-mill (Retsch) homogenization in Trizol (Invitrogen), followed by column clean-up using the RNeasy kit (QIAGEN).
RNA libraries were prepared for sequencing using standard Illumina protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Spleen
|
| Data processing |
Please refer to TSA: GATF00000000.1 (http://www.ncbi.nlm.nih.gov/nuccore/601035834)
Fragments Per Kilobase of exon per Megabase of library size (FPKM) were calculated using CLC Genomics Workbench v6.5
Genome_build: GATF00000000.1
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample ...
|
| |
|
| Submission date |
Apr 10, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Ben F Koop |
| E-mail(s) |
bkoop@uvic.ca
|
| Phone |
(250) 472-4067
|
| Organization name |
The University of Victoria
|
| Department |
Biology
|
| Lab |
Centre for Biomedical Research
|
| Street address |
PO Box 3020 STN CSC
|
| City |
Victoria |
| State/province |
BC |
| ZIP/Postal code |
V8W 3N5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL18557 |
| Series (1) |
|
| Relations |
| BioSample |
SAMN02724955 |
| SRA |
SRX514270 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM1366779_eluc_spleen_1.txt.gz |
230.2 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
