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| Status |
Public on Apr 11, 2014 |
| Title |
testis_1 |
| Sample type |
SRA |
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| Source name |
Testis
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| Organism |
Esox lucius |
| Characteristics |
age: juvenile
tissue: Testis
age: male
genotype: wild type
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| Extracted molecule |
total RNA |
| Extraction protocol |
All tissues for RNA-seq were extracted from a single, juvenile, male pike caught November 1, 2011 in the Portage Diversion/Assiniboine River Floodway near Portage la Prairie, MB, Canada (N50.00747 W98.38181). The animal was euthanized and then frozen on dry ice until sampling, and subsequently at -80°C. Tissues were extracted by mixer-mill (Retsch) homogenization in Trizol (Invitrogen), followed by column clean-up using the RNeasy kit (QIAGEN).
RNA libraries were prepared for sequencing using standard Illumina protocols.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Description |
Testis
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| Data processing |
Please refer to TSA: GATF00000000.1 (http://www.ncbi.nlm.nih.gov/nuccore/601035834)
Fragments Per Kilobase of exon per Megabase of library size (FPKM) were calculated using CLC Genomics Workbench v6.5
Genome_build: GATF00000000.1
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample ...
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| Submission date |
Apr 10, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Ben F Koop |
| E-mail(s) |
bkoop@uvic.ca
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| Phone |
(250) 472-4067
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| Organization name |
The University of Victoria
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| Department |
Biology
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| Lab |
Centre for Biomedical Research
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| Street address |
PO Box 3020 STN CSC
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| City |
Victoria |
| State/province |
BC |
| ZIP/Postal code |
V8W 3N5 |
| Country |
Canada |
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| Platform ID |
GPL18557 |
| Series (1) |
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| Relations |
| BioSample |
SAMN02724956 |
| SRA |
SRX514271 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM1366780_eluc_testis_1.txt.gz |
213.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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