|
| Status |
Public on May 01, 2014 |
| Title |
BMDM_untreated_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
BMDM
|
| Organism |
Mus musculus |
| Characteristics |
treatment: untreated
strain: C57BL/6
cell type: bone marrow derived macrophages
|
| Treatment protocol |
BMDMs were stimulated with 1uM ATRA and/or omentum culture supernatant for 24 h in macrophage serum free medium
|
| Growth protocol |
BMDMs were cultured in the presence of M-CSF for 7 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extraceted by RNeasy micro with Dnase treatment
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 2
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Apr 10, 2014 |
| Last update date |
May 01, 2014 |
| Contact name |
Yasutaka Okabe |
| Organization name |
Yale University School of Medicine
|
| Department |
Immunobiology
|
| Lab |
Ruslan Medzhitov
|
| Street address |
300 Cedar Street
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06520 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE56683 |
Gene induction by all trans retinoic acid (ATRA) and/or omentum culture supernatant in bone marrow derived macrophages (BMDMs) |
| GSE56711 |
Mechanism of tissue-specific functional polarization of peritoneal macrophages |
|
