|
| Status |
Public on Feb 26, 2015 |
| Title |
Ust_36h_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Bmdm cells
|
| Organism |
Mus musculus |
| Characteristics |
background strain: BALB/c
Sex: male
age: 8-12 weeks
cell type: bone marrow derive macrophage (Bmdm)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
BIOTIN
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Unstimulated 36h rep2
Replicate 2
|
| Data processing |
The data were normalised using quantile normalisation with lumi R (bioconductor)
|
| |
|
| Submission date |
Apr 11, 2014 |
| Last update date |
Feb 26, 2015 |
| Contact name |
Harukazu Suzuki |
| Organization name |
Riken yokohama, Riken center for life science technology
|
| Department |
Division of genomic technology
|
| Street address |
1-7-22 Suehiro-Cho, Tsurumi-Ku,
|
| City |
Yokohama |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE56736 |
Genome wide expession analysis of mouse bone marrow derive macrophage (Bmdm) cell stimulated with cytokine and infected with mycobacterium tuberculosis |
|
